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PMID: 2456269 Published · ppublish English Journal Article

Genetic control and properties of coli surface antigens of colonization factor antigen IV (PCF8775) of enterotoxigenic Escherichia coli.

Infection and immunity ·Vol. 56 ·No. 8 ·1988-08-00 ·Pages 1974-80

McConnell MM, Thomas LV, Willshaw GA, Smith HR, Rowe B

Abstract

Enterotoxigenic Escherichia coli producing coli surface antigen 4 (CS4), CS5, and CS6 of colonization factor antigen IV were examined. This factor was originally called putative colonization factor 8775 (PCF8775). All of the coli surface antigens were plasmid coded and were usually carried on the same plasmid as the genes coding for heat-stable toxin (ST) or heat-labile toxin (LT); thus, CS5-CS6-ST, CS6-ST, and CS6-LT plasmids were found. In strains of serotype O25:H42, the genes coding for CS4 and CS6 were on a plasmid separate from that containing the genes coding for ST and LT. CS4 and CS5 were fimbrial antigens with a subunit molecular mass of about 17.0 and 21.0 kilodaltons (kDa), respectively. CS6 was found as a single polypeptide with a molecular mass of about 14.5 kDa in strains of serotypes O25:H42, O27:H7, and O27:H20 when heated extracts were run on sodium dodecyl sulfate-polyacrylamide gels. CS6-positive extracts of strains of serogroups O148, O159, and O167 showed two bands with molecular masses between 14.5 and 16.0 kDa.

MeSH Terms
Antigens, Bacterial/genetics Antigens, Surface/genetics Bacterial Adhesion Bacterial Proteins/genetics,immunology DNA, Bacterial/genetics Enterotoxins/genetics Enzyme-Linked Immunosorbent Assay Epitopes Escherichia coli/genetics,immunology,pathogenicity Fimbriae Proteins Genes, Bacterial Immunodiffusion Molecular Weight Plasmids Serotyping
Chemicals
Antigens, Bacterial Antigens, Surface Bacterial Proteins DNA, Bacterial Enterotoxins Epitopes colonization factor antigens Fimbriae Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
McConnell M M
Division of Enteric Pathogens, Central Public Health Laboratory, London, United Kingdom.
Thomas L V
Willshaw G A
Smith H R
Rowe B
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.56.8.1974-1980.1988
Published
1988-08-00
Pages
1974-80
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC259510
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.56.8.1974-1980.1988 PMC Full Text View journal details
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