Dopaminergic stimulation of cyclic AMP accumulation and parathyroid hormone release from dispersed bovine parathyroid cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 74 ·No. 10 ·1977-10-00 ·Pages 4210-3

Brown EM, Carroll RJ, Aurbach GD

Abstract

The effects of dopaminergic agonists and antagonists have been studied in dispersed bovine parathyroid cells. Dopaminergic agonists caused a transient 20- to 40-fold increase in cellular cyclic AMP and a 2- to 3-fold increase in parathyroid hormone release. Dose-response relationships were similar for cyclic AMP accumulation and hormone release, whether studied by increasing agonist concentration or by increasing concentration of antagonist with constant agonist. The effects on the dopamine receptor could be differentiated from those of the previously characterized beta-adrenergic receptor by specific inhibitors. These results appear to represent proof with a homogeneous cell population that dopaminergic receptors linked to adenylate cyclase can regulate a secretory process mediated by cyclic AMP. This system should be useful in further studies on dopamine receptors and should provide a valid tool for determining interactions of radiolabeled ligands with such receptors.

MeSH Terms

Adrenergic beta-Agonists/pharmacology Animals Cattle Cyclic AMP/metabolism Dopamine/pharmacology,physiology Dopamine Antagonists Female Parathyroid Glands/drug effects,metabolism Parathyroid Hormone/metabolism Time Factors

Chemicals

Adrenergic beta-Agonists Dopamine Antagonists Parathyroid Hormone Cyclic AMP Dopamine

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Brown E M

Carroll R J

Aurbach G D

References (16)

16 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.74.10.4210

Published

1977-10-00

Pages

4210-3

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC431908

Subset

External Links

PubMed source DOI: 10.1073/pnas.74.10.4210 PMC Full Text View journal details

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Dopaminergic stimulation of cyclic AMP accumulation and parathyroid hormone release from dispersed bovine parathyroid cells.

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