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PMID: 2214021 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Env and Vpu proteins of human immunodeficiency virus type 1 are produced from multiple bicistronic mRNAs.

Journal of virology ·Vol. 64 ·No. 11 ·1990-11-00 ·Pages 5448-56

Schwartz S, Felber BK, Fenyö EM, Pavlakis GN

Abstract

Three size classes of human immunodeficiency virus type 1 (HIV-1) mRNAs are produced in infected cells: full-length, intermediate, and small. Here we report that the intermediate-size class of viral mRNAs is heterogeneous, consisting of at least 12 differentially spliced species. This group contains nine bicistronic mRNAs producing Env and Vpu and three mRNAs expressing only the first exon of tat. In the latter mRNAs, Env and Vpu expression is blocked by the presence of the upstream tat open reading frame. We conclude that internal initiation of translation is not the mechanism for generation of the bicistronic env mRNAs. Translation of HIV-1 mRNAs is consistent with the scanning mechanism in which Env is produced by leaky scanning from mRNAs that contain env as the second or third reading frame. Env and Vpu proteins are expressed from the same mRNAs and are coordinately regulated by Rev. This arrangement may reflect a requirement for coordinate expression of Vpu and Env.

MeSH Terms
Base Sequence Gene Expression Regulation, Viral Gene Products, env/genetics Gene Products, tat/genetics Genes, env/genetics HIV-1/genetics Human Immunodeficiency Virus Proteins Molecular Sequence Data Peptide Chain Initiation, Translational Polymerase Chain Reaction RNA, Messenger/genetics RNA, Viral/genetics Viral Regulatory and Accessory Proteins/genetics tat Gene Products, Human Immunodeficiency Virus
Chemicals
Gene Products, env Gene Products, tat Human Immunodeficiency Virus Proteins RNA, Messenger RNA, Viral Viral Regulatory and Accessory Proteins tat Gene Products, Human Immunodeficiency Virus vpu protein, Human immunodeficiency virus 1
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Schwartz S
Basic Research Program, National Cancer Institute-Frederick Cancer Research and Development Center, Maryland 21702-1201.
Felber B K
Fenyö E M
Pavlakis G N
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.64.11.5448-5456.1990
Published
1990-11-00
Pages
5448-56
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC248596
Subset
IM
Grants
NCI NIH HHS · N01-CO-74101 · United States
External Links
PubMed source DOI: 10.1128/JVI.64.11.5448-5456.1990 PMC Full Text View journal details
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