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PMID: 221669 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Membrane proteins specified by herpes simplex viruses. III. Role of glycoprotein VP7(B2) in virion infectivity.

Journal of virology ·Vol. 29 ·No. 3 ·1979-03-00 ·Pages 1149-58

Sarmiento M, Haffey M, Spear PG

Abstract

Experiments done with a temperature"sensitive mutant of herpes simplex virus type 1 (HSV-1) have revealed that one of the virisn glycoproteins, designated VP7(B2), is apparently not required for the production of enveloped virus particles, whereas it does play a critical role in virion infectivity. The mutant, designated HSV-1[HFEM]tsB5, fails to accumulate VP7(B2) at nonpermissive temperature and produces virions that lack detectable quantities of this glycoprotein and that have very low specific infectivity. The poor infectivity of the virions is most readily explained by failure of penetration into the host cell rather than by failure of adsorption to cells because it was shown that the VP7(B2)-deficient virions can bind to cells and that polyethylene glycol, an agent known to promote membrane fusion, can significantly enhance infectivity of the adsorbed virions.

MeSH Terms
Absorption Cell Line Glycoproteins/biosynthesis,genetics Mutation Simplexvirus/genetics,growth & development,metabolism Temperature Viral Proteins/biosynthesis,genetics Virus Replication
Chemicals
Glycoproteins Viral Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Sarmiento M
Haffey M
Spear P G
References (27)
27 references, click to expand
  1. The isolation and properties of a variant of Herpes simplex producing multinucleated giant cells in monolayer cultures in the presence of antibody.
    Am J Hyg. 1959 Sep;70:208-19 PMID: 14402487
  2. Cytochemistry and electron microscopy. The preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation.
    J Cell Biol. 1963 Apr;17:19-58 PMID: 13975866
  3. Studies of the determinant antigens of viable cells. I. A method, and its application in tissue culture studies, for enumeration of killed cells, based on the failure of virus multiplication following injury by cytotoxic antibody and complement.
    J Immunol. 1961 Dec;87:714-27 PMID: 14493436
  4. Type-common CP-1 antigen of herpes simplex virus is associated with a 59,000-molecular-weight envelope glycoprotein.
    J Virol. 1978 Jul;27(1):172-81 PMID: 80458
  5. Glycoproteins specified by herpes simplex virus type 1: their synthesis, processing and antigenic relatedness to HSV -2 glycoproteins.
    IARC Sci Publ. 1975;(11 Pt 1):49-61 PMID: 65316
  6. Membrane proteins specified by herpes simplex viruses. IV. Conformation of the virion glycoprotein designated VP7(B2).
    J Virol. 1979 Mar;29(3):1159-67 PMID: 221670
  7. Molecular genetics of herpes simplex virus. II. Mapping of the major viral glycoproteins and of the genetic loci specifying the social behavior of infected cells.
    J Virol. 1979 Feb;29(2):677-97 PMID: 219254
  8. Transformation of hamster embryo fibroblasts by a specific fragment of the herpes simplex virus genome.
    Cell. 1978 Nov;15(3):993-1002 PMID: 215318
  9. Cell fusion induced by herpes simplex virus is promoted and suppressed by different viral glycoproteins.
    Proc Natl Acad Sci U S A. 1977 Sep;74(9):3913-7 PMID: 198812
  10. Biogenesis of vaccinia: isolation and characterization of a surface component that elicits antibody suppressing infectivity and cell-cell fusion.
    Virology. 1976 Nov;75(1):232-41 PMID: 982851
  11. Production of mammalian somatic cell hybrids by means of polyethylene glycol treatment.
    Somatic Cell Genet. 1975 Oct;1(4):397-400 PMID: 1242069
  12. Proteins specified by herpes simplex virus. V. Purification and structural proteins of the herpesvirion.
    J Virol. 1972 Jan;9(1):143-59 PMID: 4110104
  13. Isolation of defective mutant of avian sarcoma virus.
    Proc Natl Acad Sci U S A. 1973 Dec;70(12):3493-7 PMID: 4357873
  14. A type of chick embryo cell that fails to support formation of infectious RSV.
    Virology. 1970 Jan;40(1):55-64 PMID: 4312375
  15. Identification of biological activities of paramyxovirus glycoproteins. Activation of cell fusion, hemolysis, and infectivity of proteolytic cleavage of an inactive precursor protein of Sendai virus.
    Virology. 1974 Feb;57(2):475-90 PMID: 4361457
  16. Type-specific protein in herpes simplex virus envelope reacts with neutralising antibody.
    Nature. 1974 May 24;249(455):360-1 PMID: 4135313
  17. Type specific and type common antigens in cells infected with herpes simplex virus type 1 and on the surfaces of naked and enveloped particles of the virus.
    J Gen Virol. 1974 Feb;22(2):159-69 PMID: 4132223
  18. Proteins specified by herpes simplex virus. XII. The virion polypeptides of type 1 strains.
    J Virol. 1974 Sep;14(3):640-51 PMID: 4369085
  19. Protein kinase in enveloped herpes simplex virions.
    Virology. 1972 Oct;50(1):287-90 PMID: 4343113
  20. Surface glycopeptides in the envelope of herpes simplex virions.
    Virology. 1972 Oct;50(1):277-9 PMID: 4343112
  21. Electron microscopy of herpes simplex virus. I. Entry.
    J Virol. 1968 May;2(5):507-16 PMID: 4301316
  22. Early events in herpes simplex virus infection: a radioautographic study.
    J Virol. 1969 Jul;4(1):67-74 PMID: 4309102
  23. Proteins specified by herpes simplex virus. 3. Viruses differing in their effects on the social behavior of infected cells specify different membrane glycoproteins.
    Proc Natl Acad Sci U S A. 1970 Apr;65(4):865-71 PMID: 4314902
  24. Preparation of herpes simplex virus of high titer.
    J Virol. 1968 Jan;2(1):83-4 PMID: 4316015
  25. Infectivity of herpes simplex virus particles with and without envelopes.
    Can J Microbiol. 1970 Oct;16(10):953-7 PMID: 4320720
  26. Membrane proteins specified by herpes simplex viruses. I. Identification of four glycoprotein precursors and their products in type 1-infected cells.
    J Virol. 1976 Mar;17(3):991-1008 PMID: 176453
  27. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.29.3.1149-1158.1979
Published
1979-03-00
Pages
1149-58
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC353275
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.29.3.1149-1158.1979 PMC Full Text View journal details
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