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PMID: 221908 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Solubilized and insolubilized bone morphogenetic protein.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 76 ·No. 4 ·1979-04-00 ·Pages 1828-32

Urist MR, Mikulski A, Lietze A

Abstract

A bone morphogenetic protein (BMP) obtained in solution by digestion of demineralized rabbit cortical bone matrix with bacterial collagenase retains its biologically active conformation in a neutral salt/ethylene glycol mixture. BMP may be insolubilized by coprecipitation with calcium phosphate and resolubilized by chemical extraction with a neutral salt in the same solvent mixture. Upon concanavalin A-Sepharose chromatography, BMP is bound by hydrophobic interaction and carbohydrate recognition and is recovered by elution with either alpha-methyl mannoside or ethylene glycol solvent mixture. Implants of both eluates and the extracts of the coprecipitate in double-walled diffusion chambers induce transmembrane bone morphogenesis. BMP is not species specific; rabbit BMP induces new bone formation in the rat. The present observations indicate that BMP is a glycoprotein.

MeSH Terms
Animals Biological Assay Bone and Bones/physiology Microbial Collagenase Protein Conformation Proteins/isolation & purification,physiology Rabbits Solubility
Chemicals
Proteins Microbial Collagenase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Urist M R
Mikulski A
Lietze A
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28 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.76.4.1828
Published
1979-04-00
Pages
1828-32
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC383485
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.76.4.1828 PMC Full Text View journal details
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