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PMID: 2221858 Published · ppublish English Journal Article

Novel beta-lactamase from Capnocytophaga sp.

Antimicrobial agents and chemotherapy ·Vol. 34 ·No. 8 ·1990-08-00 ·Pages 1501-4

Foweraker JE, Hawkey PM, Heritage J, Van Landuyt HW

Abstract

A novel beta-lactamase activity which confers resistance to expanded-spectrum cephalosporins and penicillins has been found in strain IC 5/21 of Capnocytophaga spp. Enzyme activity migrated at a molecular size of 38,000 daltons and at an isoelectric point of 3.6, with a minor band at 4.1. Kinetic studies suggested that it belonged to Richmond and Sykes beta-lactamase class 1c. Isoelectric focusing could be achieved only if a nonionic detergent was added to the gel, suggesting the presence of a hydrophobic enzyme akin to a membrane-bound beta-lactamase of gram-positive bacteria. The location of the gene coding for this beta-lactamase is not yet known.

MeSH Terms
Anti-Bacterial Agents/pharmacology Capnocytophaga/enzymology Drug Resistance, Microbial Indicators and Reagents Isoelectric Focusing Kinetics Microbial Sensitivity Tests Molecular Weight Plasmids beta-Lactamases/genetics,metabolism beta-Lactams
Chemicals
Anti-Bacterial Agents Indicators and Reagents beta-Lactams beta-Lactamases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Foweraker J E
Department of Microbiology, University of Leeds, United Kingdom.
Hawkey P M
Heritage J
Van Landuyt H W
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Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.34.8.1501
Published
1990-08-00
Pages
1501-4
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC171860
Subset
IM
External Links
PubMed source DOI: 10.1128/AAC.34.8.1501 PMC Full Text View journal details
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