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PMID: 22226352 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Production of de novo cardiomyocytes: human pluripotent stem cell differentiation and direct reprogramming.

Cell stem cell ·Vol. 10 ·No. 1 ·2012-01-06 ·Pages 16-28

Burridge PW, Keller G, Gold JD, Wu JC

Abstract

Cardiovascular disease is a leading cause of death worldwide. The limited capability of heart tissue to regenerate has prompted methodological developments for creating de novo cardiomyocytes, both in vitro and in vivo. Beyond uses in cell replacement therapy, patient-specific cardiomyocytes may find applications in drug testing, drug discovery, and disease modeling. Recently, approaches for generating cardiomyocytes have expanded to encompass three major sources of starting cells: human pluripotent stem cells (hPSCs), adult heart-derived cardiac progenitor cells (CPCs), and reprogrammed fibroblasts. We discuss state-of-the-art methods for generating de novo cardiomyocytes from hPSCs and reprogrammed fibroblasts, highlighting potential applications and future challenges.

MeSH Terms
Adult Cardiovascular Diseases/metabolism,therapy Cell Dedifferentiation Cell Differentiation Fibroblasts/cytology,metabolism,transplantation Humans Myocytes, Cardiac/cytology,metabolism,transplantation Pluripotent Stem Cells/cytology,metabolism,transplantation
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Burridge Paul W
Department of Medicine, Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA.
Keller Gordon
Gold Joseph D
Wu Joseph C
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Article Info
Journal
Cell stem cell
Abbr.
Cell Stem Cell
ISSN
1875-9777
DOI
10.1016/j.stem.2011.12.013
Published
2012-01-06
Pages
16-28
Language
English
Region
United States
NLM ID
101311472
PMCID
PMC3255078
Subset
IM
Grants
NIA NIH HHS · RC1 AG036142-01 · United States
NIA NIH HHS · RC1 AG036142 · United States
NIH HHS · DP2 OD004437 · United States
NHLBI NIH HHS · R33 HL089027 · United States
NIH HHS · DP2OD004437 · United States
NHLBI NIH HHS · R33 HL089027-05 · United States
NIA NIH HHS · RC1AG036142 · United States
NHLBI NIH HHS · R33HL089027 · United States
NIH HHS · DP2 OD004437-01 · United States
External Links
PubMed source DOI: 10.1016/j.stem.2011.12.013 PMC Full Text View journal details
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