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PMID: 22258747 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Artificial ubiquitylation is sufficient for sorting of a plasma membrane ATPase to the vacuolar lumen of Arabidopsis cells.

Planta ·Vol. 236 ·No. 1 ·2012-07-00 ·Pages 63-77

Herberth S, Shahriari M, Bruderek M, Hessner F, Müller B, Hülskamp M, Schellmann S

Abstract

Sorting of transmembrane proteins into the inner vesicles of multivesicular bodies for subsequent delivery to the vacuole/lysosome can be induced by attachment of a single ubiquitin or K63-linked ubiquitin chains to the cytosolic portion of the cargo in yeast and mammals. In plants, large efforts have been undertaken to elucidate the mechanisms of vacuolar trafficking of soluble proteins. Sorting of transmembrane proteins, by contrast, is still largely unexplored. As a proof of principle, that ubiquitin is involved in vacuolar sorting in plants we show that a translational fusion of a single ubiquitin to the Arabidopsis plasma membrane ATPase PMA-EGFP is sufficient to induce its endocytosis and sorting into the vacuolar lumen. Sorting of the artificial reporter is not dependent on ubiquitin chain formation, but involves ubiquitin's hydrophobic patch and can be inhibited by coexpression of a dominant-negative version of the ESCRT (endosomal sorting complex required for transport) related protein AtSKD1 (SUPPRESSOR OF K+ TRANSPORT GROWTH DEFECT1). Our results suggest that ubiquitin can in principle act as vacuolar sorting signal in plants.

MeSH Terms
Adenosine Triphosphatases/metabolism Arabidopsis/metabolism Cell Membrane/enzymology Endosomal Sorting Complexes Required for Transport/metabolism Protein Transport/physiology Signal Transduction Ubiquitin/metabolism Ubiquitinated Proteins/metabolism Ubiquitination Vacuoles/physiology
Chemicals
Endosomal Sorting Complexes Required for Transport Ubiquitin Ubiquitinated Proteins Adenosine Triphosphatases
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Herberth Stefanie
Botanical Institute III, Biocenter, University of Cologne, Zülpicher Str. 47b, 50674, Cologne, Germany.
Shahriari Mojgan
Bruderek Michael
Hessner Florian
Müller Britta
Hülskamp Martin
Schellmann Swen
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Article Info
Journal
Planta
Abbr.
Planta
ISSN
1432-2048
DOI
10.1007/s00425-012-1587-0
Published
2012-07-00
Epub
2012-00-19
Pages
63-77
Language
English
Region
Germany
NLM ID
1250576
Subset
IM
External Links
PubMed source DOI: 10.1007/s00425-012-1587-0 View journal details
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