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PMID: 22291204 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Widespread RNA 3'-end oligouridylation in mammals.

RNA (New York, N.Y.) ·Vol. 18 ·No. 3 ·2012-03-00 ·Pages 394-401

Choi YS, Patena W, Leavitt AD, McManus MT

Abstract

Nontemplated 3'-end oligouridylation of RNA occurs in many species, including humans. Unlike the familiar phenomenon of polyadenylation, nontemplated addition of uridines to RNA is poorly characterized in higher eukaryotes. Recent studies have reported nontemplated 3'-end oligouridylation of small RNAs and mRNAs. Oligouridylation is involved in many aspects of microRNA biology from biogenesis to turnover of the mature species, and it may also mark long mRNAs for degradation by promoting decapping of the protective 5'-cap structure. To determine the prevalence of oligouridylation in higher eukaryotes, we used next-generation sequencing technology to deeply examine the population of small RNAs in human cells. Our data revealed widespread nontemplated nucleotide addition to the 3' ends of many classes of RNA, with short stretches of uridine being the most frequently added nucleotide.

MeSH Terms
Animals Cell Line Humans Introns Mammals/genetics MicroRNAs/chemistry,metabolism RNA/chemistry,metabolism RNA 3' End Processing RNA Splicing Transcription Initiation Site Uridine/metabolism
Chemicals
MicroRNAs RNA Uridine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Choi Yun S
Biomedical Sciences Graduate Program, University of California, San Francisco, San Francisco, California 94143, USA.
Patena Weronika
Leavitt Andrew D
McManus Michael T
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Article Info
Journal
RNA (New York, N.Y.)
Abbr.
RNA
ISSN
1469-9001
DOI
10.1261/rna.029306.111
Published
2012-03-00
Epub
2012-00-30
Pages
394-401
Language
English
Region
United States
NLM ID
9509184
PMCID
PMC3285928
Subset
IM
Grants
NIDDK NIH HHS · P30 DK063720 · United States
NIDA NIH HHS · R01 DA026065 · United States
NIDA NIH HHS · 5R01DA026065 · United States
NIDDK NIH HHS · P30DK063720 · United States
External Links
PubMed source DOI: 10.1261/rna.029306.111 PMC Full Text View journal details
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