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PMID: 11071934 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The box C/D motif directs snoRNA 5'-cap hypermethylation.

Nucleic acids research ·Vol. 28 ·No. 22 ·2000-11-15 ·Pages 4467-73

Speckmann WA, Terns RM, Terns MP

Abstract

The 5'-cap structure of most spliceosomal small nuclear RNAs (snRNAs) and certain small nucleolar RNAs (snoRNAs) undergoes hypermethylation from a 7-methylguanosine to a 2,2, 7-trimethylguanosine structure. 5'-Cap hypermethylation of snRNAs is dependent upon a conserved sequence element known as the Sm site common to most snRNAs. Here we have performed a mutational analysis of U3 and U14 to determine the cis-acting sequences required for 5'-cap hypermethylation of Box C/D snoRNAs. We have found that both the conserved sequence elements Box C (termed C' in U3) and Box D are necessary for cap hypermethylation. Furthermore, the terminal stem structure that is formed by sequences that flank Box C (C' in U3) and Box D is also required. However, mutation of other conserved sequences has no effect on hypermethylation of the cap. Finally, the analysis of fragments of U3 and U14 RNAs indicates that the Box C/D motif, including Box C (C' in U3), Box D and the terminal stem, is capable of directing cap hypermethylation. Thus, the Box C/D motif, which is important for snoRNA processing, stability, nuclear retention, protein binding, nucleolar localization and function, is also necessary and sufficient for cap hypermethylation of these RNAs.

MeSH Terms
Animals Cell Nucleus/metabolism DNA Methylation Female Models, Molecular Mutation Oocytes RNA Caps/genetics,metabolism RNA Stability RNA, Small Nucleolar/chemistry,genetics,metabolism Regulatory Sequences, Nucleic Acid Xenopus
Chemicals
RNA Caps RNA, Small Nucleolar RNA, U3 small nucleolar
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Speckmann W A
Department of Biochemistry and Molecular Biology, Life Science Building, University of Georgia, Athens, GA 30602, USA.
Terns R M
Terns M P
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
1362-4962
DOI
10.1093/nar/28.22.4467
Published
2000-11-15
Pages
4467-73
Language
English
Region
England
NLM ID
0411011
PMCID
PMC113864
Subset
IM
Grants
NIGMS NIH HHS · R01 GM054682 · United States
NIGMS NIH HHS · GM54682 · United States
External Links
PubMed source DOI: 10.1093/nar/28.22.4467 PMC Full Text View journal details
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