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PMID: 7556076 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Base pairing between U3 and the pre-ribosomal RNA is required for 18S rRNA synthesis.

The EMBO journal ·Vol. 14 ·No. 17 ·1995-09-01 ·Pages 4350-6

Beltrame M, Tollervey D

Abstract

The nucleolus, the site of pre-ribosomal RNA (pre-rRNA) synthesis and processing in eukaryotic cells, contains a number of small nucleolar RNAs (snoRNAs). Yeast U3 snoRNA is required for the processing of 18S rRNA from larger precursors and contains a region complementary to the pre-rRNA. Substitution mutations in the pre-rRNA which disrupt this base pairing potential are lethal and prevent synthesis of 18S rRNA. These mutant pre-rRNAs show defects in processing which closely resemble the effects of genetic depletion of components of the U3 snoRNP. Co-expression of U3 snoRNAs which carry compensatory mutations allows the mutant pre-rRNAs to support viability and synthesize 18S rRNA at high levels. Pre-rRNA processing steps which are blocked by the external transcribed spacer region mutations are largely restored by expression of the compensatory U3 mutants. Pre-rRNA processing therefore requires direct base pairing between snoRNA and the substrate. Base pairing with the substrate is thus a common feature of small RNAs involved in mRNA and rRNA maturation.

MeSH Terms
Base Composition Base Sequence Blotting, Northern DNA Primers Molecular Sequence Data Mutagenesis Polymerase Chain Reaction RNA Precursors/chemistry,metabolism RNA, Messenger/biosynthesis RNA, Ribosomal, 18S/biosynthesis RNA, Small Nuclear/chemistry,metabolism Saccharomyces cerevisiae/genetics,metabolism
Chemicals
DNA Primers RNA Precursors RNA, Messenger RNA, Ribosomal, 18S RNA, Small Nuclear
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Beltrame M
Gene Expression Programme, European Molecular Biology Laboratory, Heidelberg, Germany.
Tollervey D
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33 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1995-09-01
Pages
4350-6
Language
English
Region
England
NLM ID
8208664
PMCID
PMC394519
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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