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PMID: 8413202 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A U3 small nuclear ribonucleoprotein-requiring processing event in the 5' external transcribed spacer of Xenopus precursor rRNA.

Molecular and cellular biology ·Vol. 13 ·No. 10 ·1993-10-00 ·Pages 5990-8

Mougey EB, Pape LK, Sollner-Webb B

Abstract

A processing site has been identified within the 5' external transcribed spacer (ETS) of Xenopus laevis and X. borealis pre-RNAs, and this in vivo processing can be reproduced in vitro. It involves a stable and specific association of the pre-rRNA with factors in the cell extract, including at least four RNA-contacting polypeptides, yielding a distinct complex that sediments at 20S. Processing also requires the U3 small nuclear RNA. This processing, at residue +105 of the 713-nucleotide X. laevis 5' ETS, is highly reminiscent of the initial processing cleavage of mouse pre-rRNA within its 3.5-kb 5' ETS, previously thought to be mammal specific. The frog and mouse processing signals share a short essential sequence motif, and mouse factors can faithfully process the frog pre-rRNA. This conservation suggests that this 5' ETS processing site serves an evolutionarily selective function.

MeSH Terms
Animals Base Sequence Biological Evolution Conserved Sequence DNA Humans Mice Molecular Sequence Data RNA Precursors/metabolism RNA Processing, Post-Transcriptional RNA, Ribosomal/metabolism Ribonucleoproteins, Small Nuclear/metabolism Transcription, Genetic Xenopus laevis
Chemicals
RNA Precursors RNA, Ribosomal Ribonucleoproteins, Small Nuclear DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Mougey E B
Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
Pape L K
Sollner-Webb B
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.13.10.5990-5998.1993
Published
1993-10-00
Pages
5990-8
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC364653
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.13.10.5990-5998.1993 PMC Full Text View journal details
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