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PMID: 8078771 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A common core structure for U3 small nucleolar RNAs.

Nucleic acids research ·Vol. 22 ·No. 16 ·1994-08-25 ·Pages 3354-64

Hartshorne T, Agabian N

Abstract

U3 nucleolar small RNA (snRNA) is involved in early processing of the primary rRNA transcript. A secondary structure model for the unusually small Trypanosoma brucei U3 snRNA was deduced by chemical modification and enzymatic cleavage of U3 snRNA in deproteinized and ribonucleoprotein (RNP) forms. Comprehensive alignment of U3 snRNAs from vertebrate, plant, fungal and protozoan species clearly delineated conserved and divergent features. The 5' domain of the T. brucei U3 snRNA appears to form one small, flexible 5' stem loop structure followed by a long single-stranded region; this model is a variation on 5' domain structures proposed for other U3 snRNAs which do not conform to a single model. The 3' domain of T. brucei U3 snRNA contains four single-stranded sequences conserved between U3 snRNAs. Of these, structural probing determined that the configurations of GAU region and box B and C sequences are altered by protein interactions in U3 snRNP. Conspicuously, the 3' domains of trypanosomal U3 snRNAs lack stem loops II and III, indicating that these structures are not required for conserved U3 snRNA functions.

MeSH Terms
Animals Base Sequence Endoribonucleases/metabolism Molecular Sequence Data Nucleic Acid Conformation Phylogeny RNA, Small Nuclear/chemistry Ribonuclease T1/metabolism Ribonuclease, Pancreatic/metabolism Ribonucleoproteins, Small Nuclear/chemistry Sequence Homology, Nucleic Acid Trypanosoma brucei brucei/genetics
Chemicals
RNA, Small Nuclear Ribonucleoproteins, Small Nuclear Endoribonucleases ribonuclease V(1) Ribonuclease T1 Ribonuclease, Pancreatic
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hartshorne T
Intercampus Program in Molecular Parasitology, School of Pharmacy, University of California, San Francisco 94143-1204.
Agabian N
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48 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/22.16.3354
Published
1994-08-25
Pages
3354-64
Language
English
Region
England
NLM ID
0411011
PMCID
PMC523729
Subset
IM
Grants
NIAID NIH HHS · AI21975 · United States
Databases
GENBANK
S74818, S74819
External Links
PubMed source DOI: 10.1093/nar/22.16.3354 PMC Full Text View journal details
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