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PMID: 8417321 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

RNA B is the major nucleolar trimethylguanosine-capped small nuclear RNA associated with fibrillarin and pre-rRNAs in Trypanosoma brucei.

Molecular and cellular biology ·Vol. 13 ·No. 1 ·1993-01-00 ·Pages 144-54

Hartshorne T, Agabian N

Abstract

RNA B is one of three abundant trimethylguanosine-capped U small nuclear RNAs (snRNAs) of Trypanosoma brucei which is not strongly identified with other U snRNAs by sequence homology. We show here that RNA B is a highly diverged U3 snRNA homolog likely involved in pre-rRNA processing. Sequence identity between RNA B and U3 snRNAs is limited; only two of four boxes of homology conserved between U3 snRNAs are obvious in RNA B. These are the box A homology, specific for U3 snRNAs, and the box C homology, common to nucleolar snRNAs and required for association with the nucleolar protein, fibrillarin. A 35-kDa T. brucei fibrillarin homolog was identified by using an anti-Physarum fibrillarin monoclonal antibody. RNA B and fibrillarin were localized in nucleolar fractions of the nucleus which contained pre-rRNAs and did not contain nucleoplasmic snRNAs. Fibrillarin and RNA B were precipitated by scleroderma patient serum S4, which reacts with fibrillarins from diverse organisms; RNA B was the only trimethylguanosine-capped RNA precipitated. Furthermore, RNA B sedimented with pre-rRNAs in nondenaturing sucrose gradients, similarly to U3 and other nucleolar snRNAs, suggesting that RNA B is hydrogen bonded to rRNA intermediates and might be involved in their processing.

MeSH Terms
Animals Base Sequence Cell Compartmentation Cell Nucleolus/metabolism Chromosomal Proteins, Non-Histone/metabolism Hydrogen Bonding Molecular Sequence Data Nucleic Acid Conformation Nucleic Acid Precursors/genetics Oligodeoxyribonucleotides/chemistry Precipitin Tests RNA Caps/metabolism RNA Processing, Post-Transcriptional RNA, Protozoan/genetics RNA, Ribosomal/genetics RNA, Small Nuclear/metabolism Trypanosoma brucei brucei/genetics
Chemicals
Chromosomal Proteins, Non-Histone Nucleic Acid Precursors Oligodeoxyribonucleotides RNA Caps RNA, Protozoan RNA, Ribosomal RNA, Small Nuclear fibrillarin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hartshorne T
Intercampus Program in Molecular Parasitology, School of Pharmacy, University of California, San Francisco 94143-1204.
Agabian N
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.13.1.144-154.1993
Published
1993-01-00
Pages
144-54
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC358894
Subset
IM
Grants
PHS HHS · A121975 · United States
External Links
PubMed source DOI: 10.1128/mcb.13.1.144-154.1993 PMC Full Text View journal details
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