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PMID: 2233728 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A phorbol ester-regulated ribonuclease system controlling transforming growth factor beta 1 gene expression in hematopoietic cells.

Molecular and cellular biology ·Vol. 10 ·No. 11 ·1990-11-00 ·Pages 5983-90

Wager RE, Assoian RK

Abstract

12-Tetradecanoylphorbol-13-acetate (TPA)-induced differentiation of U937 promonocytes leads to a 30-fold increase in transforming growth factor beta 1 (TGF-beta 1) gene expression, and this effect results from a stabilized mRNA. Similar up-regulation was detected in TPA-treated K562 erythroblasts but was absent from cell lines that do not differentiate in response to TPA. Related studies in vitro showed that postnuclear extracts of U937 promonocytes contain a ribonuclease system that degrades TGF-beta 1 mRNA selectively and that this system is completely blocked by prior treatment of the cells with TPA. These data identify a new mechanism for regulating TGF-beta 1 mRNA levels and allow us to establish the overall basis for control of TGF-beta 1 gene expression by activation of protein kinase C. Our results also provide a new basis for understanding the long-term up-regulation of TGF-beta 1 gene expression that can accompany hematopoietic cell differentiation.

MeSH Terms
Cell Differentiation/drug effects Cell Line Gene Expression Regulation/drug effects Humans Kinetics Models, Genetic Monocytes Polyribosomes/metabolism RNA, Messenger/genetics,metabolism Ribonucleases/metabolism Tetradecanoylphorbol Acetate/pharmacology Transcription, Genetic/drug effects Transforming Growth Factor beta/genetics
Chemicals
RNA, Messenger Transforming Growth Factor beta Ribonucleases Tetradecanoylphorbol Acetate
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Wager R E
Department of Biochemistry and Molecular Biophysics, College of Physicians and Surgeons, Columbia University, New York, New York 10032.
Assoian R K
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.10.11.5983-5990.1990
Published
1990-11-00
Pages
5983-90
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC361396
Subset
IM
Grants
NHLBI NIH HHS · HL38884 · United States
NIDDK NIH HHS · T32DK07328 · United States
External Links
PubMed source DOI: 10.1128/mcb.10.11.5983-5990.1990 PMC Full Text View journal details
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