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PMID: 22385961 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Spindle pole bodies exploit the mitotic exit network in metaphase to drive their age-dependent segregation.

Cell ·Vol. 148 ·No. 5 ·2012-03-02 ·Pages 958-72

Hotz M, Leisner C, Chen D, Manatschal C, Wegleiter T, Ouellet J, Lindstrom D, Gottschling DE, Vogel J, Barral Y

Abstract

Like many asymmetrically dividing cells, budding yeast segregates mitotic spindle poles nonrandomly between mother and daughter cells. During metaphase, the spindle positioning protein Kar9 accumulates asymmetrically, localizing specifically to astral microtubules emanating from the old spindle pole body (SPB) and driving its segregation to the bud. Here, we show that the SPB component Nud1/centriolin acts through the mitotic exit network (MEN) to specify asymmetric SPB inheritance. In the absence of MEN signaling, Kar9 asymmetry is unstable and its preference for the old SPB is disrupted. Consistent with this, phosphorylation of Kar9 by the MEN kinases Dbf2 and Dbf20 is not required to break Kar9 symmetry but is instead required to maintain stable association of Kar9 with the old SPB throughout metaphase. We propose that MEN signaling links Kar9 regulation to SPB identity through biasing and stabilizing the age-insensitive, cyclin-B-dependent mechanism of symmetry breaking.

MeSH Terms
Cell Cycle Proteins/metabolism Deoxyribonucleases/metabolism Metaphase Saccharomyces cerevisiae/cytology,metabolism Saccharomyces cerevisiae Proteins/metabolism Spindle Apparatus/metabolism tRNA Methyltransferases/metabolism
Chemicals
Cell Cycle Proteins Saccharomyces cerevisiae Proteins tRNA Methyltransferases Deoxyribonucleases TRM2 protein, S cerevisiae
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Hotz Manuel
Institute of Biochemistry, Biology Department, ETH Zurich, 8093 Zurich, Switzerland.
Leisner Christian
Chen Daici
Manatschal Cristina
Wegleiter Thomas
Ouellet Jimmy
Lindstrom Derek
Gottschling Dan E
Vogel Jackie
Barral Yves
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Article Info
Journal
Cell
Abbr.
Cell
ISSN
1097-4172
DOI
10.1016/j.cell.2012.01.041
Published
2012-03-02
Pages
958-72
Language
English
Region
United States
NLM ID
0413066
PMCID
PMC3779431
Subset
IM
Grants
NCI NIH HHS · P30 CA015704 · United States
NIA NIH HHS · R01 AG023779 · United States
CIHR · MOP-64404 · Canada
NIA NIH HHS · AG023779 · United States
NIA NIH HHS · R37 AG023779 · United States
External Links
PubMed source DOI: 10.1016/j.cell.2012.01.041 PMC Full Text View journal details
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