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PMID: 19154724 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Cell polarity determinants establish asymmetry in MEN signaling.

Developmental cell ·Vol. 16 ·No. 1 ·2009-01-00 ·Pages 132-45

Monje-Casas F, Amon A

Abstract

Components of the mitotic exit network (MEN), a signaling pathway that triggers exit from mitosis, localize to the spindle pole body (SPB) that migrates into the daughter cell during anaphase but are largely absent from the SPB that remains in the mother cell. Through the analysis of one of the determinants of this asymmetry, Bfa1, we find that the machinery responsible for establishing cell polarity and cytoplasmic microtubules collaborate to establish MEN asymmetry. In cells defective in the Cdc42 signaling pathway or the formin Bni1, Bfa1 localizes to both SPBs. The quantitative analysis of Bfa1 localization further shows that Bfa1 can associate with both SPBs in a transient and highly dynamic fashion, but the protein is stabilized on the SPB that migrates into the daughter cell during anaphase through microtubule-bud cortex interactions. Our results indicate that mother-daughter cell asymmetry determinants establish MEN signaling asymmetry through microtubule-bud cortex interactions.

MeSH Terms
Actins/metabolism Cell Cycle/physiology Cell Cycle Proteins/genetics,metabolism Cell Polarity/physiology Cytoskeletal Proteins/genetics,metabolism Cytoskeleton/metabolism Microtubules/metabolism Monomeric GTP-Binding Proteins/genetics,metabolism Recombinant Fusion Proteins/genetics,metabolism Saccharomyces cerevisiae/cytology,genetics,metabolism Saccharomyces cerevisiae Proteins/genetics,metabolism Signal Transduction/physiology Spindle Apparatus/metabolism Tubulin/genetics,metabolism
Chemicals
Actins BFA1 protein, S cerevisiae BUB2 protein, S cerevisiae Cell Cycle Proteins Cytoskeletal Proteins Recombinant Fusion Proteins Saccharomyces cerevisiae Proteins TEM1 protein, S cerevisiae Tubulin Monomeric GTP-Binding Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Monje-Casas Fernando
David H. Koch Institute for Integrative Cancer Research and Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Amon Angelika
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Article Info
Journal
Developmental cell
Abbr.
Dev Cell
ISSN
1878-1551
DOI
10.1016/j.devcel.2008.11.002
Published
2009-01-00
Pages
132-45
Language
English
Region
United States
NLM ID
101120028
PMCID
PMC2713012
Subset
IM
Grants
Howard Hughes Medical Institute · United States
NIGMS NIH HHS · R29 GM056800 · United States
NIGMS NIH HHS · R01 GM056800 · United States
NIGMS NIH HHS · GM056800 · United States
NIGMS NIH HHS · R01 GM056800-14 · United States
External Links
PubMed source DOI: 10.1016/j.devcel.2008.11.002 PMC Full Text View journal details
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