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PMID: 9114012 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Two active states of the Ras-related Bud1/Rsr1 protein bind to different effectors to determine yeast cell polarity.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 94 ·No. 9 ·1997-04-29 ·Pages 4463-8

Park HO, Bi E, Pringle JR, Herskowitz I

Abstract

Cells of budding yeast organize their cytoskeleton in a highly polarized manner during vegetative growth. Selection of a site for polarization requires a group of proteins including a Ras-like GTPase, Bud1, and its regulators. Another group of proteins, which includes a Rho-like GTPase (Cdc42), its guanine nucleotide exchange factor (Cdc24), and Bem1, is necessary for organization of the actin cytoskeleton and for cell polarization. We have proposed previously that the Bud1 protein, through its GTPase cycle, determines the localization of one or more of the cell polarity proteins to the bud site. Herein we demonstrate that Bud1 directly interacts with Cdc24 and Bem1: Bud1 in its GTP-bound form associates preferentially with Cdc24, whereas the GDP-bound form of Bud1 associates with Bem1. We also present subcellular fractionation data for Bud1 that is consistent with the idea that Bud1 can travel between the site for budding on the plasma membrane and the cytosol. We propose that Bud1 can exist in two active states for association with different partners and that the switch from Bud1-GTP to Bud1-GDP provides a regulatory device for ordered assembly of a macromolecular complex at the bud site.

MeSH Terms
Adaptor Proteins, Signal Transducing Cell Compartmentation Cell Cycle Proteins/metabolism Cell Polarity/physiology Cytoplasm/chemistry Fungal Proteins/metabolism GTP Phosphohydrolases/genetics,metabolism Guanine Nucleotide Exchange Factors Membranes/chemistry Models, Biological Mutation Protein Binding Proto-Oncogene Proteins/metabolism Saccharomyces cerevisiae/physiology Saccharomyces cerevisiae Proteins Signal Transduction rab GTP-Binding Proteins
Chemicals
Adaptor Proteins, Signal Transducing CDC24 protein, S cerevisiae Cell Cycle Proteins Fungal Proteins Guanine Nucleotide Exchange Factors Proto-Oncogene Proteins Saccharomyces cerevisiae Proteins BEM1 protein, S cerevisiae GTP Phosphohydrolases RSR1 protein, S cerevisiae rab GTP-Binding Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Park H O
Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94143-0448, USA. [email protected]
Bi E
Pringle J R
Herskowitz I
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.94.9.4463
Email
[email protected]
Published
1997-04-29
Pages
4463-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC20745
Subset
IM
Grants
NIGMS NIH HHS · R01 GM031006 · United States
NIGMS NIH HHS · R37 GM031006 · United States
NIGMS NIH HHS · GM31006 · United States
NIGMS NIH HHS · GM48052 · United States
External Links
PubMed source DOI: 10.1073/pnas.94.9.4463 PMC Full Text View journal details
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