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PMID: 2254017 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Polymorphic expression of defensins in neutrophils from outbred rats.

Infection and immunity ·Vol. 58 ·No. 12 ·1990-12-00 ·Pages 3899-902

Eisenhauer P, Harwig SS, Szklarek D, Ganz T, Lehrer RI

Abstract

We isolated and characterized a rat neutrophil defensin, RatNP-2, that differs from the previously described defensin RatNP-1 by containing Ser-7 in place of Arg-7. Although the resulting charge difference rendered RatNP-2 easily distinguishable from RatNP-1 on polyacrylamide gel electrophoresis gels, the two defensins exhibited very similar antimicrobial efficacies against Salmonella typhimurium, Staphylococcus aureus, and Candida albicans. The polymorphonuclear leukocytes of Sprague-Dawley rats obtained from one of two breeders also showed a marked polymorphism for defensin RatNP-4. This defensin was absent in two of seven animals and present in 1x or 2x relative amounts in the others. These observations indicate that a striking degree of defensin polymorphism exists in the polymorphonuclear leukocytes of outbred rodents.

MeSH Terms
Amino Acids/analysis Animals Blood Bactericidal Activity Blood Proteins/analysis,pharmacology Defensins Electrophoresis, Polyacrylamide Gel Female Neutrophils/immunology Polymorphism, Genetic Rabbits Rats Salmonella typhimurium/drug effects
Chemicals
Amino Acids Blood Proteins Defensins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Eisenhauer P
Department of Medicine, UCLA Center for the Health Sciences 90024.
Harwig S S
Szklarek D
Ganz T
Lehrer R I
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.58.12.3899-3902.1990
Published
1990-12-00
Pages
3899-902
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC313752
Subset
IM
Grants
NIAID NIH HHS · AI22839 · United States
NIAID NIH HHS · AI29595 · United States
NHLBI NIH HHS · HL35640 · United States
External Links
PubMed source DOI: 10.1128/iai.58.12.3899-3902.1990 PMC Full Text View journal details
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