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PMID: 226064 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Regulation of ketogenesis during the suckling-weanling transition in the rat. Studies with isolated hepatocytes.

The Biochemical journal ·Vol. 180 ·No. 1 ·1979-04-15 ·Pages 137-44

Benito M, Whitelaw E, Williamson DH

Abstract

The rates of ketogenesis from endogenous substrates, butyrate or oleate, have been measured in isolated hepatocytes from suckling and weanling rats. Ketogenesis from endogenous substrate and from oleate decreased on weaning, whereas the rate from butyrate remained unchanged. It is concluded that the major site of regulation of ketogenesis during this period of development involves the disposal of long-chain fatty acyl-CoA between the esterification and beta-oxidation pathways. Modulators of lipogenesis [dihydroxyacetone and 5-(tetradecyloxy)-2-furoic acid] did not alter the rate of ketogenesis in hepatocytes from suckling rats, and it is suggested that this is due to the low rate of lipogenesis in these cells. Hepatocytes from fed weanling rats have a high rate of lipogenesis and evidence is presented for a reciprocal relationship between ketogenesis and lipogenesis, and ketogenesis, and esterification in these cells. Dibutyryl cyclic AMP stimulated ketogenesis from oleate in hepatocytes from fed weanling rats, even in the presence of an inhibitor of lipogenesis [5-(tetradecyloxy)-2-furoic acid], but not in cells from suckling rats. It is suggested that cyclic AMP may act via inhibition of esterification and that in hepatocytes from suckling rats ketogenesis is already maximally stimulated by the high basal concentrations of cyclic AMP [Beaudry, Chiasson & Exton (1977) Am. J. Physiol. 233, E175--E180].

MeSH Terms
Animals Animals, Suckling Bucladesine/pharmacology Carboxylic Acids/pharmacology Dihydroxyacetone/pharmacology Esterification Furans/pharmacology Hypolipidemic Agents/pharmacology In Vitro Techniques Ketone Bodies/biosynthesis Lipids/biosynthesis Liver/cytology,growth & development,metabolism Rats Weaning
Chemicals
Carboxylic Acids Furans Hypolipidemic Agents Ketone Bodies Lipids 5-(tetradecyloxy)-2-furancarboxylic acid Bucladesine Dihydroxyacetone
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Benito M
Whitelaw E
Williamson D H
References (44)
44 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1800137
Published
1979-04-15
Pages
137-44
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1161028
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1800137 PMC Full Text View journal details
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