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PMID: 22837360 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Effects of drought on gene expression in maize reproductive and leaf meristem tissue revealed by RNA-Seq.

Plant physiology ·Vol. 160 ·No. 2 ·2012-10-00 ·Pages 846-67

Kakumanu A, Ambavaram MM, Klumas C, Krishnan A, Batlang U, Myers E, Grene R, Pereira A

Abstract

Drought stress affects cereals especially during the reproductive stage. The maize (Zea mays) drought transcriptome was studied using RNA-Seq analysis to compare drought-treated and well-watered fertilized ovary and basal leaf meristem tissue. More drought-responsive genes responded in the ovary compared with the leaf meristem. Gene Ontology enrichment analysis revealed a massive decrease in transcript abundance of cell division and cell cycle genes in the drought-stressed ovary only. Among Gene Ontology categories related to carbohydrate metabolism, changes in starch and Suc metabolism-related genes occurred in the ovary, consistent with a decrease in starch levels, and in Suc transporter function, with no comparable changes occurring in the leaf meristem. Abscisic acid (ABA)-related processes responded positively, but only in the ovaries. Related responses suggested the operation of low glucose sensing in drought-stressed ovaries. The data are discussed in the context of the susceptibility of maize kernel to drought stress leading to embryo abortion and the relative robustness of dividing vegetative tissue taken at the same time from the same plant subjected to the same conditions. Our working hypothesis involves signaling events associated with increased ABA levels, decreased glucose levels, disruption of ABA/sugar signaling, activation of programmed cell death/senescence through repression of a phospholipase C-mediated signaling pathway, and arrest of the cell cycle in the stressed ovary at 1 d after pollination. Increased invertase levels in the stressed leaf meristem, on the other hand, resulted in that tissue maintaining hexose levels at an "unstressed" level, and at lower ABA levels, which was correlated with successful resistance to drought stress.

MeSH Terms
Abscisic Acid/pharmacology Adaptation, Physiological Cell Cycle Checkpoints Cell Death Cell Division Droughts Flowers/genetics,metabolism,physiology Gene Expression Regulation, Plant Genes, Plant Glucose/metabolism Meristem/genetics,metabolism Phenotype Plant Leaves/genetics,metabolism,physiology RNA Splice Sites RNA, Plant/genetics Seeds/genetics,metabolism Sequence Analysis, RNA Signal Transduction Stress, Physiological Transcriptome Type C Phospholipases/genetics,metabolism Zea mays/genetics,metabolism,physiology
Chemicals
RNA Splice Sites RNA, Plant Abscisic Acid Type C Phospholipases Glucose
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Kakumanu Akshay
Virginia Bioinformatics Institute , Virginia Tech, Blacksburg, Virginia 24061, USA.
Ambavaram Madana M R
Klumas Curtis
Krishnan Arjun
Batlang Utlwang
Myers Elijah
Grene Ruth
Pereira Andy
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Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
1532-2548
DOI
10.1104/pp.112.200444
Published
2012-10-00
Epub
2012-00-26
Pages
846-67
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC3461560
Subset
IM
External Links
PubMed source DOI: 10.1104/pp.112.200444 PMC Full Text View journal details
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