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PMID: 21844310 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The Arabidopsis bZIP gene AtbZIP63 is a sensitive integrator of transient abscisic acid and glucose signals.

Plant physiology ·Vol. 157 ·No. 2 ·2011-10-00 ·Pages 692-705

Matiolli CC, Tomaz JP, Duarte GT, Prado FM, Del Bem LE, Silveira AB, Gauer L, Corrêa LG, Drumond RD, Viana AJ, Di Mascio P, Meyer C, Vincentz M

Abstract

Glucose modulates plant metabolism, growth, and development. In Arabidopsis (Arabidopsis thaliana), Hexokinase1 (HXK1) is a glucose sensor that may trigger abscisic acid (ABA) synthesis and sensitivity to mediate glucose-induced inhibition of seedling development. Here, we show that the intensity of short-term responses to glucose can vary with ABA activity. We report that the transient (2 h/4 h) repression by 2% glucose of AtbZIP63, a gene encoding a basic-leucine zipper (bZIP) transcription factor partially involved in the Snf1-related kinase KIN10-induced responses to energy limitation, is independent of HXK1 and is not mediated by changes in ABA levels. However, high-concentration (6%) glucose-mediated repression appears to be modulated by ABA, since full repression of AtbZIP63 requires a functional ABA biosynthetic pathway. Furthermore, the combination of glucose and ABA was able to trigger a synergistic repression of AtbZIP63 and its homologue AtbZIP3, revealing a shared regulatory feature consisting of the modulation of glucose sensitivity by ABA. The synergistic regulation of AtbZIP63 was not reproduced by an AtbZIP63 promoter-5'-untranslated region::β-glucuronidase fusion, thus suggesting possible posttranscriptional control. A transcriptional inhibition assay with cordycepin provided further evidence for the regulation of mRNA decay in response to glucose plus ABA. Overall, these results indicate that AtbZIP63 is an important node of the glucose-ABA interaction network. The mechanisms by which AtbZIP63 may participate in the fine-tuning of ABA-mediated abiotic stress responses according to sugar availability (i.e., energy status) are discussed.

MeSH Terms
5' Untranslated Regions Abscisic Acid/biosynthesis,metabolism Arabidopsis Proteins/genetics,metabolism Basic-Leucine Zipper Transcription Factors/genetics,metabolism Biosynthetic Pathways Gene Expression Regulation, Plant Glucose/metabolism Glucuronidase/genetics,metabolism Hexokinase/metabolism Promoter Regions, Genetic Protein Serine-Threonine Kinases/metabolism RNA Stability Signal Transduction Trans-Activators/metabolism
Chemicals
5' Untranslated Regions Arabidopsis Proteins Basic-Leucine Zipper Transcription Factors Sen1 protein, Arabidopsis Trans-Activators bZIP34 protein, Arabidopsis bZIP63 protein, Arabidopsis Abscisic Acid SNF1-related protein kinases Hexokinase ATHXK1 protein, Arabidopsis Protein Serine-Threonine Kinases Glucuronidase Glucose
Authors & Affiliations
13 authors, click to expand affiliations / ORCID
Matiolli Cleverson Carlos
Centro de Biologia Molecular e Engenharia Genética, Universidade Estadual de Campinas, Cidade Universitária Zeferino Vaz, CP6010 Campinas, Sao Paulo, Brazil.
Tomaz Juarez Pires
Duarte Gustavo Turqueto
Prado Fernanda Manso
Del Bem Luiz Eduardo Vieira
Silveira Amanda Bortolini
Gauer Luciane
Corrêa Luiz Gustavo Guedes
Drumond Rodrigo Duarte
Viana Américo José Carvalho
Di Mascio Paolo
Meyer Christian
Vincentz Michel
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Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
1532-2548
DOI
10.1104/pp.111.181743
Published
2011-10-00
Epub
2011-00-15
Pages
692-705
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC3192551
Subset
IM
External Links
PubMed source DOI: 10.1104/pp.111.181743 PMC Full Text View journal details
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