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PMID: 16377568 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, U.S. Gov't, Non-P.H.S.

Endogenous siRNAs derived from a pair of natural cis-antisense transcripts regulate salt tolerance in Arabidopsis.

Cell ·Vol. 123 ·No. 7 ·2005-12-29 ·Pages 1279-91

Borsani O, Zhu J, Verslues PE, Sunkar R, Zhu JK

Abstract

In higher eukaryotes, miRNAs and siRNAs guide translational inhibition, mRNA cleavage, or chromatin regulation. We found that the antisense overlapping gene pair of Delta(1)-pyrroline-5-carboxylate dehydrogenase (P5CDH), a stress-related gene, and SRO5, a gene of unknown function, generates two types of siRNAs. When both transcripts are present, a 24-nt siRNA is formed by a biogenesis pathway dependent on DCL2, RDR6, SGS3, and NRPD1A. Initial cleavage of the P5CDH transcript guided by the 24-nt siRNA establishes a phase for the subsequent generation of 21-nt siRNAs by DCL1 and further cleavage of P5CDH transcripts. The expression of SRO5 is induced by salt, and this induction is required to initiate siRNA formation. Our data suggest that the P5CDH and SRO5 proteins are also functionally related, and that the P5CDH-SRO5 gene pair defines a mode of siRNA function and biogenesis that may be applied to other natural cis-antisense gene pairs in eukaryotic genomes.

MeSH Terms
1-Pyrroline-5-Carboxylate Dehydrogenase/genetics,metabolism Arabidopsis/drug effects,genetics,physiology Arabidopsis Proteins/genetics,metabolism Base Sequence Down-Regulation/drug effects,physiology Models, Biological Molecular Sequence Data Mutation Proline/metabolism RNA, Antisense/genetics RNA, Small Interfering/biosynthesis,physiology Salts/metabolism,pharmacology Up-Regulation/drug effects,physiology
Chemicals
Arabidopsis Proteins RNA, Antisense RNA, Small Interfering Salts Proline 1-Pyrroline-5-Carboxylate Dehydrogenase ALDH12A1 protein, Arabidopsis
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Borsani Omar
Institute for Integrative Genome Biology and Department of Botany and Plant Sciences, University of California, Riverside, CA 92521, USA.
Zhu Jianhua
Verslues Paul E
Sunkar Ramanjulu
Zhu Jian-Kang
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Article Info
Journal
Cell
Abbr.
Cell
ISSN
0092-8674
DOI
10.1016/j.cell.2005.11.035
Published
2005-12-29
Pages
1279-91
Language
English
Region
United States
NLM ID
0413066
PMCID
PMC3137516
Subset
IM
Grants
NIGMS NIH HHS · R01 GM059138 · United States
NIGMS NIH HHS · R01 GM059138-11 · United States
NIGMS NIH HHS · R01GM0707501 · United States
NIGMS NIH HHS · R01GM59138 · United States
External Links
PubMed source DOI: 10.1016/j.cell.2005.11.035 PMC Full Text View journal details
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