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PMID: 2296082 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Mutational analysis of the human immunodeficiency virus type 2 (HIV-2) genome in relation to HIV-1 and simian immunodeficiency virus SIV (AGM).

Journal of virology ·Vol. 64 ·No. 2 ·1990-02-00 ·Pages 742-7

Shibata R, Miura T, Hayami M, Ogawa K, Sakai H, Kiyomasu T, Ishimoto A, Adachi A

Abstract

We constructed an infectious molecular clone of the human immunodeficiency virus type 2 (HIV-2) and generated nine frameshift mutants corresponding to nine open reading frames identified so far. Three structural (gag, pol, env) and two regulative (tat, rev) gene mutants were not infectious, whereas vif, vpx, vpr, and nef genes were dispensable for infectivity. All of the mutants except env and rev were cytopathic in CD4+ human leukemia cells. In transfection assays, the expression of HIV-2 long terminal repeat was activated by infectious clones of HIV-1, HIV-2, and simian immunodeficiency virus from African green monkey but not by the tat mutants. However, an HIV-2 tat mutant could produce small amounts of virus proteins and particles in contrast to a rev mutant, which directed no detectable synthesis of virus proteins and virions.

MeSH Terms
Blotting, Northern Blotting, Western Cell Line Chloramphenicol O-Acetyltransferase/genetics DNA, Viral/genetics Genes, Viral HIV-1/genetics HIV-2/genetics Humans Leukemia Mutation Plasmids RNA, Viral/genetics Restriction Mapping Simian Immunodeficiency Virus/genetics Transcriptional Activation Transfection
Chemicals
DNA, Viral RNA, Viral Chloramphenicol O-Acetyltransferase
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Shibata R
Department of Viral Oncology, Kyoto University, Japan.
Miura T
Hayami M
Ogawa K
Sakai H
Kiyomasu T
Ishimoto A
Adachi A
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.64.2.742-747.1990
Published
1990-02-00
Pages
742-7
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC249168
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.64.2.742-747.1990 PMC Full Text View journal details
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