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PMID: 2300570 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Oncogenic transformation by the tax gene of human T-cell leukemia virus type I in vitro.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 87 ·No. 3 ·1990-02-00 ·Pages 1071-5

Tanaka A, Takahashi C, Yamaoka S, Nosaka T, Maki M, Hatanaka M

Abstract

Human T-cell leukemia virus type I (HTLV-I) is a causative agent of adult T-cell leukemia (ATL). To elucidate the role of HTLV-I in leukemogenesis, we examined the biological activity of a defective HTLV-I provirus with the env-pX 3' long terminal repeat region cloned from leukemic cells of an ATL patient. Transfection experiments showed growth stimulation of NIH 3T3 cells--growing beyond the saturation density and growing in soft agar. Since the pX sequence is known to encode three proteins, Tax, Rex, and p21x, the biological activity of each pX gene was examined separately. The growth-stimulating activity was induced only by the tax gene in NIH 3T3 cells and Rat-1 cells. Furthermore, the tax gene induced tumorigenicity in nude mice when introduced into Rat-1 cells. Thus, a transcriptional transactivator gene of HTLV-I, tax, is clearly identified as a viral oncogene without a cellular homolog. The transforming activity of tax, possibly via a transcriptional deregulation of cell growth control, may play an important role in leukemogenesis of ATL in addition to its aberrant stimulation of the interleukin 2 system.

MeSH Terms
Animals Cell Division Cell Line Cell Transformation, Neoplastic Cells, Cultured Genes, Viral Human T-lymphotropic virus 1/genetics Humans Leukemia-Lymphoma, Adult T-Cell/microbiology Mice Plasmids Rats Trans-Activators/genetics Transfection Viral Structural Proteins/genetics
Chemicals
Trans-Activators Viral Structural Proteins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Tanaka A
Institute for Virus Research, Kyoto University, Japan.
Takahashi C
Yamaoka S
Nosaka T
Maki M
Hatanaka M
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44 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.87.3.1071
Published
1990-02-00
Pages
1071-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC53412
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.87.3.1071 PMC Full Text View journal details
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