Home LiteratureArticle Details
PMID: 2300587 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

A cluster of hematopoietic serine protease genes is found on the same chromosomal band as the human alpha/delta T-cell receptor locus.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 87 ·No. 3 ·1990-02-00 ·Pages 960-3

Hanson RD, Hohn PA, Popescu NC, Ley TJ

Abstract

The chymotrypsin-like family of serine protease genes includes several members that are expressed exclusively in subsets of hematopoietic cells. For example, human neutrophil elastase and cathepsin G are expressed only in myelomonocytic precursors, and cytotoxic-T-cell serine proteases are found only in cytotoxic lymphocytes. We have used a cathepsin G cDNA probe to clone two cathepsin G-like genes (designated CGL-1 and CGL-2) from a human genomic library. We have determined that CGL-1 is identical to a previously identified gene (known as CCPI, CTLA I, or cytotoxic serine protease B) that is expressed only in activated cytotoxic T lymphocytes. We show here that cathepsin G, CGL-1, and CGL-2 are linked on an approximately 50-kilobase locus found on human chromosome 14 at band q11.2. This gene cluster maps to the same chromosomal band as the alpha and delta T-cell receptor genes; this region is involved in most chromosomal translocations and inversions that are specifically associated with T-cell malignancies.

MeSH Terms
Blotting, Southern Cathepsin G Cathepsins/genetics Chromosome Banding Chromosome Mapping Chromosomes, Human, Pair 14 Cosmids Genetic Linkage Genome, Human Hematopoietic Stem Cells/enzymology Humans Macromolecular Substances Receptors, Antigen, T-Cell/genetics Restriction Mapping Serine Endopeptidases/genetics
Chemicals
Macromolecular Substances Receptors, Antigen, T-Cell Cathepsins Serine Endopeptidases CTSG protein, human Cathepsin G
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Hanson R D
Department of Medicine, Jewish Hospital at Washington University Medical Center, St. Louis, MO 63110.
Hohn P A
Popescu N C
Ley T J
References (37)
37 references, click to expand
  1. Genomic organization and chromosomal localization of the human cathepsin G gene.
    J Biol Chem. 1989 Aug 15;264(23):13412-9 PMID: 2569462
  2. Molecular analysis of the human beta-globin locus activation region.
    Proc Natl Acad Sci U S A. 1989 Jul;86(14):5439-43 PMID: 2748594
  3. Elastase and cathepsin G of human monocytes. Quantification of cellular content, release in response to stimuli, and heterogeneity in elastase-mediated proteolytic activity.
    J Immunol. 1989 Nov 1;143(9):2961-8 PMID: 2681419
  4. Human T-cell antigen receptor (TCR) delta-chain locus and elements responsible for its deletion are within the TCR alpha-chain locus.
    Proc Natl Acad Sci U S A. 1988 Dec;85(24):9694-8 PMID: 2974161
  5. cDNA clones from autocrine thymic lymphoma cells encode two mitogenic proteins, a serine protease and a truncated T-cell receptor beta-chain.
    Oncogene Res. 1988;3(4):301-12 PMID: 2976140
  6. The beta-globin dominant control region activates homologous and heterologous promoters in a tissue-specific manner.
    Cell. 1989 Mar 24;56(6):969-77 PMID: 2924354
  7. Expression of the neutrophil elastase gene during human bone marrow cell differentiation.
    J Exp Med. 1989 Mar 1;169(3):833-45 PMID: 2538548
  8. Gene rearrangements and translocations in lymphoproliferative diseases.
    Blood. 1989 May 1;73(6):1402-15 PMID: 2653455
  9. Immunofluorescent localization of a serine protease in rat small intestine.
    Proc Natl Acad Sci U S A. 1978 Jun;75(6):2785-9 PMID: 351615
  10. Elastase of U-937 monocytelike cells. Comparisons with elastases derived from human monocytes and neutrophils and murine macrophagelike cells.
    J Clin Invest. 1982 Feb;69(2):384-93 PMID: 6915940
  11. Evolutionary conservation in the untranslated regions of actin mRNAs: DNA sequence of a human beta-actin cDNA.
    Nucleic Acids Res. 1984 Feb 10;12(3):1687-96 PMID: 6322116
  12. Cathepsin G in human mononuclear phagocytes: comparisons between monocytes and U937 monocyte-like cells.
    J Immunol. 1984 May;132(5):2547-51 PMID: 6562154
  13. Gene for alpha-chain of human T-cell receptor: location on chromosome 14 region involved in T-cell neoplasms.
    Science. 1985 Mar 1;227(4690):1044-7 PMID: 3919442
  14. Induction of chromosome banding by trypsin/EDTA for gene mapping by in situ hybridization.
    Cytogenet Cell Genet. 1985;39(1):73-4 PMID: 3920012
  15. The "beta-like-globin" gene domain in human erythroid cells.
    Proc Natl Acad Sci U S A. 1985 Oct;82(19):6384-8 PMID: 3879975
  16. A developmentally stable chromatin structure in the human beta-globin gene cluster.
    Proc Natl Acad Sci U S A. 1986 Mar;83(5):1359-63 PMID: 3456593
  17. Cloning of two genes that are specifically expressed in activated cytotoxic T lymphocytes.
    Proc Natl Acad Sci U S A. 1986 Mar;83(5):1448-52 PMID: 3485287
  18. 12-o-Tetradecanoyl-phorbol-13-acetate-differentiated U937 cells express a macrophage-like profile of neutral proteinases. High levels of secreted collagenase and collagenase inhibitor accompany low levels of intracellular elastase and cathepsin G.
    J Clin Invest. 1986 May;77(5):1675-81 PMID: 3009552
  19. Cloning of a cDNA for a T cell-specific serine protease from a cytotoxic T lymphocyte.
    Science. 1986 May 16;232(4752):854-8 PMID: 2422755
  20. The inducible cytotoxic T-lymphocyte-associated gene transcript CTLA-1 sequence and gene localization to mouse chromosome 14.
    Nature. 1986 Jul 17-23;322(6076):268-71 PMID: 3090449
  21. Cloning of the mast cell protease, RMCP II. Evidence for cell-specific expression and a multi-gene family.
    J Biol Chem. 1987 Apr 15;262(11):5377-84 PMID: 3549719
  22. A family of serine esterases in lytic granules of cytolytic T lymphocytes.
    Cell. 1987 Jun 5;49(5):679-85 PMID: 3555842
  23. Induction of mRNA for a serine protease and a beta-thromboglobulin-like protein in mitogen-stimulated human leukocytes.
    J Immunol. 1987 Jul 1;139(1):250-6 PMID: 2953813
  24. Characterization of a new human diploid myeloid leukemia cell line (PLB-985) with granulocytic and monocytic differentiating capacity.
    Blood. 1987 Aug;70(2):372-8 PMID: 3475136
  25. Position-independent, high-level expression of the human beta-globin gene in transgenic mice.
    Cell. 1987 Dec 24;51(6):975-85 PMID: 3690667
  26. Orthogonal-field-alternation gel electrophoresis.
    Methods Enzymol. 1987;155:468-82 PMID: 3431469
  27. Identification and sequencing of cDNA clones encoding the granule-associated serine proteases granzymes D, E, and F of cytolytic T lymphocytes.
    Proc Natl Acad Sci U S A. 1988 Jul;85(13):4814-8 PMID: 3260382
  28. T-cell antigen receptor genes and T-cell recognition.
    Nature. 1988 Aug 4;334(6181):395-402 PMID: 3043226
  29. The molecular evolution of genes and proteins: a tale of two serines.
    Nature. 1988 Aug 11;334(6182):528-30 PMID: 3136396
  30. Selective expression of an antigen receptor on CD8-bearing T lymphocytes in transgenic mice.
    Nature. 1988 Sep 15;335(6187):271-4 PMID: 3261843
  31. Molecular cloning of an inducible serine esterase gene from human cytotoxic lymphocytes.
    Proc Natl Acad Sci U S A. 1988 Sep;85(18):6924-8 PMID: 3261871
  32. Proximity of the CTLA-1 serine esterase and Tcr alpha loci in mouse and man.
    Immunogenetics. 1988;28(6):439-44 PMID: 3182016
  33. Genomic organization of the human T-cell antigen-receptor alpha/delta locus.
    Proc Natl Acad Sci U S A. 1988 Nov;85(21):8166-70 PMID: 3186718
  34. Clustering of breakpoints on chromosome 10 in acute T-cell leukemias with the t(10;14) chromosome translocation.
    Proc Natl Acad Sci U S A. 1989 Jun;86(11):4161-5 PMID: 2542965
  35. Isolation of single-copy human genes from a library of yeast artificial chromosome clones.
    Science. 1989 Jun 16;244(4910):1348-51 PMID: 2544027
  36. The t(11;14)(p15;q11) in a T-cell acute lymphoblastic leukemia cell line activates multiple transcripts, including Ttg-1, a gene encoding a potential zinc finger protein.
    Mol Cell Biol. 1989 May;9(5):2124-32 PMID: 2501659
  37. Genomic organization and chromosomal assignment for a serine protease gene (CSPB) expressed by human cytotoxic lymphocytes.
    Genomics. 1989 Jul;5(1):110-7 PMID: 2788607
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.87.3.960
Published
1990-02-00
Pages
960-3
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC53389
Subset
IM
Grants
NCI NIH HHS · CA49712 · United States
NIDDK NIH HHS · DK38682 · United States
External Links
PubMed source DOI: 10.1073/pnas.87.3.960 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2