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PMID: 2312721 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Experimental non-O group 1 Vibrio cholerae gastroenteritis in humans.

The Journal of clinical investigation ·Vol. 85 ·No. 3 ·1990-03-00 ·Pages 697-705

Morris JG, Takeda T, Tall BD, Losonsky GA, Bhattacharya SK, Forrest BD, Kay BA, Nishibuchi M

Abstract

In this study, 27 volunteers received one of three non-O group 1 Vibrio cholerae strains in doses as high as 10(9) CFU. Only one strain (strain C) caused diarrhea: this strain was able to colonize the gastrointestinal tract, and produced a heat-stable enterotoxin (NAG-ST). Diarrhea was not seen with a strain (strain A) that colonized the intestine but did not produce NAG-ST, nor with a strain (strain B) that produced NAG-ST but did not colonize. Persons receiving strain C had diarrhea and abdominal cramps. Diarrheal stool volumes ranged from 154 to 5,397 ml; stool samples from the patient having 5,397 ml of diarrhea were tested and found to contain NAG-ST. The median incubation period for illness was 10 h. There was a suggestion that occurrence of diarrhea was dependent on inoculum size. Immune responses to homologous outer membrane proteins, lipopolysaccharide, and whole-cell lysates were demonstrable with all three strains. Our data demonstrate that V. cholerae of O groups other than 1 are able to cause severe diarrheal disease. However, not all strains are pathogenic for humans: virulence of strain C may be dependent on its ability both to colonize the intestine and to produce a toxin such as NAG-ST.

MeSH Terms
Adolescent Adult Amino Acid Sequence Animals Antibodies, Bacterial/analysis Cholera Toxin/biosynthesis Enterotoxins/analysis,toxicity Female Gastroenteritis/etiology Humans Male Molecular Sequence Data Rabbits Vibrio cholerae/immunology,pathogenicity Virulence
Chemicals
Antibodies, Bacterial Enterotoxins stN protein, Vibrio cholerae Cholera Toxin
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Morris J G
Department of Medicine, University of Maryland School of Medicine, Baltimore 21201.
Takeda T
Tall B D
Losonsky G A
Bhattacharya S K
Forrest B D
Kay B A
Nishibuchi M
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Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI114494
Published
1990-03-00
Pages
697-705
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC296485
Subset
IM
Grants
NIAID NIH HHS · 1R22AI28856-01 · United States
NIAID NIH HHS · N01-AI-62528 · United States
External Links
PubMed source DOI: 10.1172/JCI114494 PMC Full Text View journal details
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