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PMID: 2342468 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Transcription of the procyclic acidic repetitive protein genes of Trypanosoma brucei.

Molecular and cellular biology ·Vol. 10 ·No. 6 ·1990-06-00 ·Pages 3036-47

Clayton CE, Fueri JP, Itzhaki JE, Bellofatto V, Sherman DR, Wisdom GS, Vijayasarathy S, Mowatt MR

Abstract

The procyclic acidic repetitive protein (parp) genes of Trypanosoma brucei encode a small family of abundant surface proteins whose expression is restricted to the procyclic form of the parasite. They are found at two unlinked loci, parpA and parpB; transcription of both loci is developmentally regulated. The region of homology upstream of the A and B parp genes is only 640 base pairs long and may contain sequences responsible for transcriptional initiation and regulation. Transcription upstream of this putative promoter region is not developmentally regulated and is much less active than that of the parp genes; the polymerase responsible is inhibited by alpha-amanitin, whereas that transcribing the parp genes is not. Transcription of the parp genes is strongly stimulated by low levels of UV irradiation. The putative parp promoter, when placed upstream of the chloramphenicol acetyltransferase gene, is sufficient to cause production of chloramphenicol acetyltransferase in a T. brucei DNA transformation assay. Taken together, these results suggest that a promoter for an alpha-amanitin-resistant RNA polymerase lies less than 600 nucleotides upstream of the parp genes.

MeSH Terms
Animals Antigens, Protozoan/genetics Base Sequence Cell Nucleus/metabolism Chromosome Deletion Cloning, Molecular Dose-Response Relationship, Radiation Membrane Glycoproteins Molecular Sequence Data Plasmids Polymerase Chain Reaction Promoter Regions, Genetic Protozoan Proteins Restriction Mapping Sequence Homology, Nucleic Acid Transcription, Genetic/radiation effects Transfection Trypanosoma brucei brucei/genetics Ultraviolet Rays Variant Surface Glycoproteins, Trypanosoma/genetics
Chemicals
Antigens, Protozoan Membrane Glycoproteins Protozoan Proteins Variant Surface Glycoproteins, Trypanosoma procyclic acidic repetitive protein, Trypanosoma
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Clayton C E
Rockefeller University, New York, New York 10021-6399.
Fueri J P
Itzhaki J E
Bellofatto V
Sherman D R
Wisdom G S
Vijayasarathy S
Mowatt M R
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37 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.10.6.3036-3047.1990
Published
1990-06-00
Pages
3036-47
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC360668
Subset
IM
Grants
NIAID NIH HHS · AI21729 · United States
NIAID NIH HHS · AI22229 · United States
Databases
GENBANK
M33129, M33130
External Links
PubMed source DOI: 10.1128/mcb.10.6.3036-3047.1990 PMC Full Text View journal details
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