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PMID: 2779575 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Trypanosoma brucei: enrichment by UV of intergenic transcripts from the variable surface glycoprotein gene expression site.

Molecular and cellular biology ·Vol. 9 ·No. 9 ·1989-09-00 ·Pages 4022-5

Coquelet H, Tebabi P, Pays A, Steinert M, Pays E

Abstract

The expression site for the variable surface glycoprotein (VSG) gene AnTat 1.3A of Trypanosoma brucei is 45 kilobases long and encompasses seven expression site-associated genes (ESAGs) (E. Pays, P. Tebabi, A. Pays, H. Coquelet, P. Revelard, D. Salmon, and M. Steinert, Cell 57:835-845, 1989). After UV irradiation, several large transcripts from the putative promoter region were strongly enriched. We report that one such major transcript starts near the poly(A) addition site of the first gene (ESAG 7), spans the intergenic region, and extends to the poly(A) addition site of the second gene (ESAG 6), thus bypassing the normal 3' splice site of the ESAG 6 mRNA. Since this transcript is spliced, we conclude that UV irradiation does not inhibit splicing but stabilizes unstable processing products. This demonstrates that at least some intergenic regions of the VSG gene expression site are continuously transcribed in accordance with a polycistronic transcription model.

MeSH Terms
Animals Base Sequence DNA/genetics Gene Expression Regulation/radiation effects Molecular Sequence Data RNA Processing, Post-Transcriptional/radiation effects RNA Splicing/radiation effects Transcription, Genetic/radiation effects Trypanosoma brucei brucei/genetics,radiation effects Ultraviolet Rays Variant Surface Glycoproteins, Trypanosoma/genetics
Chemicals
Variant Surface Glycoproteins, Trypanosoma DNA
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Coquelet H
Department of Molecular Biology, University of Brussels, Rhode-Saint-Genèse, Belgium.
Tebabi P
Pays A
Steinert M
Pays E
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.9.9.4022-4025.1989
Published
1989-09-00
Pages
4022-5
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC362465
Subset
IM
Databases
GENBANK
M28494, M28495
External Links
PubMed source DOI: 10.1128/mcb.9.9.4022-4025.1989 PMC Full Text View journal details
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