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PMID: 2357966 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Oct-4: a germline-specific transcription factor mapping to the mouse t-complex.

The EMBO journal ·Vol. 9 ·No. 7 ·1990-07-00 ·Pages 2185-95

Schöler HR, Dressler GR, Balling R, Rohdewohld H, Gruss P

Abstract

Oct-4 is a maternally expressed octamer-binding protein encoded by the murine Oct-4 gene. It is present in unfertilized oocytes, but also in the inner cell mass and in primordial germ cells. Here we show that the ectopic expression of Oct-4 in HeLa cells is sufficient for transcriptional activation from the octamer motif, indicating that Oct-4 is a transcription factor. Therefore, Oct-4 is the first transcription factor described that is specific for the early stages of mouse development. The spatial and temporal expression patterns were further determined using in situ hybridization. With this technique Oct-4 expression is detected in the oocyte, in the blastocyst and before gastrulation in the embryonic ectoderm. After day 8 Oct-4 expression decreases and is restricted to primordial germ cells from about day 8.5 onwards. Therefore Oct-4 is a transcription factor that is specifically expressed in cells participating in the generation of the germline lineage. Linkage analysis using B X D recombinant inbred mouse strains demonstrates that Oct-4 maps to chromosome 17 in or near the major histocompatibility complex. Several mouse mutants in the distal region of the mouse t-complex affecting blastocyst and embryonic ectoderm formation also map to this region.

MeSH Terms
Animals Base Sequence Blastocyst/physiology Chromosome Mapping DNA Probes DNA-Binding Proteins/genetics Female Gene Expression Genetic Vectors HeLa Cells/metabolism Major Histocompatibility Complex Male Mice Mice, Inbred Strains Molecular Sequence Data Nucleic Acid Hybridization Octamer Transcription Factor-3 Oocytes/physiology Promoter Regions, Genetic Recombination, Genetic Restriction Mapping Transcription Factors/genetics
Chemicals
DNA Probes DNA-Binding Proteins Octamer Transcription Factor-3 Pou5f1 protein, mouse Transcription Factors
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Schöler H R
Max-Planck-Institute for Biophysical Chemistry, Department of Molecular Cell Biology, Göttingen, FRG.
Dressler G R
Balling R
Rohdewohld H
Gruss P
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1990-07-00
Pages
2185-95
Language
English
Region
England
NLM ID
8208664
PMCID
PMC551941
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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