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PMID: 2377625 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

K-sam, an amplified gene in stomach cancer, is a member of the heparin-binding growth factor receptor genes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 87 ·No. 15 ·1990-08-00 ·Pages 5983-7

Hattori Y, Odagiri H, Nakatani H, Miyagawa K, Naito K, Sakamoto H, Katoh O, Yoshida T, Sugimura T, Terada M

Abstract

DNA fragments amplified in a stomach cancer-derived cell line, KATO-III, were previously identified by the in-gel DNA renaturation method, and a 0.2-kilobase-pair fragment of the amplified sequence was subsequently cloned. By genomic walking, a portion of the exon of the gene flanking this 0.2-kilobase-pair fragment was cloned, and the gene was designated as K-sam (KATO-III cell-derived stomach cancer amplified gene). The K-sam cDNAs, corresponding to the 3.5-kilobase K-sam mRNA, were cloned from the KATO-III cells. Sequence analysis revealed that this gene coded for 682 amino acid residues that satisfied the characteristics of the receptor tyrosine kinase. The K-sam gene had significant homologies with bek, FLG, and chicken basic fibroblast growth factor receptor gene. The K-sam gene was amplified in KATO-III cells with the major transcript of 3.5-kilobases in size. This gene was also expressed in some other stomach cancer cells, a small cell lung cancer, and germ cell tumors.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Blotting, Southern Cell Line DNA, Neoplasm/genetics Filaggrin Proteins Gene Amplification Growth Substances/metabolism Humans Mice Mice, Nude Molecular Sequence Data Multigene Family Neoplasm Transplantation RNA, Neoplasm/genetics,isolation & purification Receptors, Mitogen/genetics Receptors, Vascular Endothelial Growth Factor Sequence Homology, Nucleic Acid Stomach Neoplasms/genetics Transplantation, Heterologous
Chemicals
DNA, Neoplasm FLG protein, human Filaggrin Proteins Growth Substances RNA, Neoplasm Receptors, Mitogen Receptors, Vascular Endothelial Growth Factor
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Hattori Y
Genetics Division, National Cancer Center Research Institute, Tokyo, Japan.
Odagiri H
Nakatani H
Miyagawa K
Naito K
Sakamoto H
Katoh O
Yoshida T
Sugimura T
Terada M
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.87.15.5983
Published
1990-08-00
Pages
5983-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC54454
Subset
IM
Databases
GENBANK
M35718
External Links
PubMed source DOI: 10.1073/pnas.87.15.5983 PMC Full Text View journal details
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