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PMID: 237853 Published · ppublish English Comparative Study Journal Article

Cytochemical discrimination between catalases and peroxidases using diaminobenzidine.

Histochemistry ·Vol. 41 ·No. 4 ·1975-00-00 ·Pages 281-312

Roels F, Wisse E, De Prest B, van der Meulen J

Abstract

The influence on diaminobenzidine staining of four variables: prefixation in aldehyde, temperature and pH of incubation, and H2O2 concentration, was investigated in catalase-, as well as in peroxydase-containing material. Catalase from five different sources and five types of peroxidase were examined. It is concluded: (a) when cells are incubated without prior fixation, in a DAB medium at room temperature and pH 7.3 with 0.003% H2O2, peroxidases produce a visible cytochemical stain, while catalases do not; (b) the cytochemical reaction elicited by catalases is stimulated by prior aldehyde fixation in specified conditions, and incubation at 45 degrees C and pH 9.7 with 0.06% H2O2; (c) under the latter circumstances several peroxidases also stain. Ultrastructural preservation is satisfactory in tissues incubated prior to fixation.

MeSH Terms
Aminobiphenyl Compounds Animals Benzidines Buffers Catalase/analysis Diamines Female Frozen Sections Glutaral Histocytochemistry Humans Hydrogen Peroxide Hydrogen-Ion Concentration Male Mice Microbodies/enzymology Peroxidases/analysis Rats Temperature
Chemicals
Aminobiphenyl Compounds Benzidines Buffers Diamines Hydrogen Peroxide Peroxidases Catalase Glutaral
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Roels F
Wisse E
De Prest B
van der Meulen J
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129 references, click to expand
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Article Info
Journal
Histochemistry
Abbr.
Histochemistry
ISSN
0301-5564
DOI
10.1007/BF00490073
Published
1975-00-00
Pages
281-312
Language
English
Region
Germany
NLM ID
0411300
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00490073
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