Thermal denaturation profiles and gel mobility shift analysis of oligodeoxynucleotide triplexes.

Nucleic acids research ·Vol. 18 ·No. 16 ·1990-08-25 ·Pages 4859-66

Shea RG, Ng P, Bischofberger N

Abstract

Oligodeoxypurine- and oligodeoxypyrimidine-containing strands were mixed under conditions conducive to the formation of triple stranded assemblies. The mixtures were characterized both by their UV absorbance change with increasing temperature and by their mobility in non-denaturing polyacrylamide gels. Duplexes 34 bp long containing 15 central purines on one strand and 15 complementary pyrimidines on the other strand yielded new melting transitions and showed different gel mobilities upon combination with oligopyrimidine 15-mers. The dependence of the thermal denaturation profiles on pH, salt concentration, GC content, strand orientation, base mismatches, and strand length was investigated.

MeSH Terms

Base Composition Base Sequence Electrophoresis, Polyacrylamide Gel Hydrogen-Ion Concentration Molecular Sequence Data Nucleic Acid Denaturation Oligodeoxyribonucleotides Sodium Chloride Spectrophotometry, Ultraviolet Temperature

Chemicals

Oligodeoxyribonucleotides Sodium Chloride

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Shea R G

Genentech, Inc., Department of Molecular Biology, South San Francisco, CA 94080.

Ng P

Bischofberger N

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26 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/18.16.4859

Published

1990-08-25

Pages

4859-66

Language

English

Region

England

NLM ID

0411011

PMCID

PMC331964

Subset

External Links

PubMed source DOI: 10.1093/nar/18.16.4859 PMC Full Text View journal details

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Thermal denaturation profiles and gel mobility shift analysis of oligodeoxynucleotide triplexes.

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