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PMID: 2398542 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

CD4 immunoadhesin, but not recombinant soluble CD4, blocks syncytium formation by human immunodeficiency virus type 2-infected lymphoid cells.

Journal of virology ·Vol. 64 ·No. 10 ·1990-10-00 ·Pages 5194-8

Sekigawa I, Chamow SM, Groopman JE, Byrn RA

Abstract

Recombinant soluble CD4 (rCD4) has been shown to be an effective inhibitor of human immunodeficiency virus type 1 (HIV-1) and HIV-2 infection of lymphoid cells in vitro. In this report, we characterized the effects of rCD4, the V1V2 fragment of CD4, and the immunoadhesin CD4-immunoglobulin G on syncytium formation between lymphoid cells infected by HIV-1 or HIV-2 and uninfected cells. All three molecules blocked HIV-1-mediated syncytium formation, but only CD4-immunoglobulin G blocked HIV-2-mediated syncytium formation. rCD4 and the V1V2 fragment of CD4 enhanced HIV-2-mediated syncytium formation. These results suggest that the process of cell fusion is significantly different between HIV-1- and HIV-2-infected cells.

MeSH Terms
CD4 Antigens/genetics,immunology Cell Line Cell Survival Giant Cells/cytology,immunology HIV-1/genetics,immunology HIV-2/genetics,immunology Humans Immunoglobulin G Recombinant Proteins/immunology T-Lymphocytes/cytology,immunology
Chemicals
CD4 Antigens Immunoglobulin G Recombinant Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Sekigawa I
Department of Medicine, New England Deaconess Hospital, Harvrd Medical School, Boston, Massachusetts 02215.
Chamow S M
Groopman J E
Byrn R A
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.64.10.5194-5198.1990
Published
1990-10-00
Pages
5194-8
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC248017
Subset
IM
Grants
NHLBI NIH HHS · HL33774 · United States
NHLBI NIH HHS · HL42112 · United States
NHLBI NIH HHS · HL43510 · United States
External Links
PubMed source DOI: 10.1128/JVI.64.10.5194-5198.1990 PMC Full Text View journal details
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