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PMID: 2403927 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The U6 gene of Saccharomyces cerevisiae is transcribed by RNA polymerase C (III) in vivo and in vitro.

The EMBO journal ·Vol. 9 ·No. 1 ·1990-01-00 ·Pages 271-7

Moenne A, Camier S, Anderson G, Margottin F, Beggs J, Sentenac A

Abstract

Unlike the majority of genes encoding small nuclear RNAs, which are transcribed by RNA polymerase B, the U6 gene contains features found in both class B and class C genes, indicating the involvement of a combination of transcription factors normally specific to each class of genes. We present direct genetic and biochemical evidence that the U6 gene of Saccharomyces cerevisiae is transcribed by RNA polymerase C in vivo as well as in vitro. A mutant strain with a temperature-sensitive defect in the large subunit of RNA polymerase C that results in defective transcription of tRNA and 5S RNA genes shows a corresponding defect in U6 RNA levels. Also, purified RNA polymerase C transcribes the U6 gene when supplemented with partially purified TFIIIB. The other class C transcription factors, TFIIIA and Tau (TFIIIC), are not required in this system.

MeSH Terms
Base Sequence DNA-Directed RNA Polymerases/metabolism Molecular Sequence Data Mutation Nucleic Acid Hybridization RNA Polymerase III/metabolism RNA, Ribosomal, 5S/genetics RNA, Small Nuclear/genetics RNA, Transfer/genetics Restriction Mapping Saccharomyces cerevisiae/genetics Single-Strand Specific DNA and RNA Endonucleases Transcription Factor TFIIIB Transcription Factors/pharmacology Transcription, Genetic
Chemicals
RNA, Ribosomal, 5S RNA, Small Nuclear Transcription Factor TFIIIB Transcription Factors RNA, Transfer DNA-Directed RNA Polymerases RNA Polymerase III Single-Strand Specific DNA and RNA Endonucleases
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Moenne A
Département de Biologie, CEN de Saclay, Commissariat à l'Energie Atomique, Gif sur Yvette, France.
Camier S
Anderson G
Margottin F
Beggs J
Sentenac A
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1990-01-00
Pages
271-7
Language
English
Region
England
NLM ID
8208664
PMCID
PMC551658
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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