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PMID: 3302945 Published · ppublish English Journal Article

The requirement for the A block promoter element in tRNA gene transcription in vitro depends on the ionic environment.

Nucleic acids research ·Vol. 15 ·No. 14 ·1987-07-24 ·Pages 5699-713

Gabrielsen OS, Oyen TB

Abstract

When yeast cell extracts that faithfully transcribe class III genes are provided with different electrolyte ions, the pattern of transcripts changes. A transcription unit in pBR322, silent with 0.1M potassium chloride, becomes active in the presence of 0.1M potassium acetate. This pseudogene depends on transcription factors B and C and RNA polymerase III like a tRNA gene. The transcribed region contains the only sequence in pBR322 homologous to the modified B block consensus sequence GTTCRDNNC found in normal tRNA genes. The presence of a block A sequence is less evident. When a block A deleted tRNA(GLU) gene was constructed, it behaved similarly: poorly transcribed with 0.1M potassium chloride, well transcribed with 0.1M potassium acetate. In fact, the deletion of the A block promoter element from the tRNA(GLU) gene did not dramatically lower its transcription when tested with potassium acetate, while it had a strong negative effect when tested with potassium chloride. Consequently the requirement for this promoter element is not constant but is a function of the electrolyte composition.

MeSH Terms
Base Sequence Cloning, Molecular Electrolytes Genes, Fungal Genetic Vectors Ions Plasmids Promoter Regions, Genetic RNA, Transfer/genetics RNA, Transfer, Amino Acyl/genetics Saccharomyces cerevisiae/genetics Transcription, Genetic
Chemicals
Electrolytes Ions RNA, Transfer, Amino Acyl RNA, Transfer
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Gabrielsen O S
Oyen T B
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49 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/15.14.5699
Published
1987-07-24
Pages
5699-713
Language
English
Region
England
NLM ID
0411011
PMCID
PMC306016
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/15.14.5699 PMC Full Text View journal details
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