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PMID: 6269065 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Sequence of a yeast DNA fragment containing a chromosomal replicator and a tRNA Glu 3 gene.

Nucleic acids research ·Vol. 9 ·No. 12 ·1981-06-25 ·Pages 2949-59

Feldmann H, Olah J, Friedenreich H

Abstract

The sequence of a 1.9 kb Bam x Hind III fragment from yeast has been determined. This fragment is part of a yeast 6.7 kb Hind III segment cloned into pBR322 (pY20). The fragment carries a single gene for a glutamate tRNA which has no intron. According to genetic analyses [1] this fragment also contains a yeast chromosomal replicator. We have analyzed the sequence for potential open reading frames and for several structural features which are thought to be involved in the initiation of DNA replication. Hybridization studies have revealed that portions of this sequence are repeated within the yeast genome.

MeSH Terms
Base Sequence Chromosomes/physiology Cloning, Molecular DNA Restriction Enzymes DNA, Fungal/genetics DNA, Recombinant Genes Glutamates/genetics Nucleic Acid Hybridization Plasmids RNA, Transfer, Amino Acyl/genetics Saccharomyces cerevisiae/genetics
Chemicals
DNA, Fungal DNA, Recombinant Glutamates RNA, Transfer, Amino Acyl DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Feldmann H
Olah J
Friedenreich H
References (23)
23 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/9.12.2949
Published
1981-06-25
Pages
2949-59
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326904
Subset
IM
Databases
GENBANK
V01337
External Links
PubMed source DOI: 10.1093/nar/9.12.2949 PMC Full Text View journal details
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