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PMID: 6253952 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Structure of a yeast non-initiating methionine-tRNA gene.

Nucleic acids research ·Vol. 8 ·No. 9 ·1980-05-10 ·Pages 1975-86

Olah J, Feldmann H

Abstract

4 to 8 kb Hind III fragments of yeast DNA were cloned into pBR322. One of these clones (pY6m3) containing a single tRNA3Met gene has been characterized in detail. The DNA sequence of the structural gene is colinear with the tRNA sequence, which means that in this case no intervening sequence is present. The 5'-leader and 3'-trailer sequences have also been determined. The 5'-flanking region can be folded up into possible secondary structures.

MeSH Terms
Base Sequence Cloning, Molecular DNA Restriction Enzymes Genes Nucleic Acid Conformation RNA, Fungal/genetics RNA, Transfer, Amino Acyl/genetics Saccharomyces cerevisiae/genetics
Chemicals
RNA, Fungal RNA, Transfer, Amino Acyl tRNA(m)(Met), methionine- DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Olah J
Feldmann H
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31 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/8.9.1975
Published
1980-05-10
Pages
1975-86
Language
English
Region
England
NLM ID
0411011
PMCID
PMC324051
Subset
IM
Databases
GENBANK
J01372
External Links
PubMed source DOI: 10.1093/nar/8.9.1975 PMC Full Text View journal details
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