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PMID: 6329678 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A novel method for site-directed mutagenesis: its application to an eukaryotic tRNAPro gene promoter.

The EMBO journal ·Vol. 1 ·No. 4 ·1982-00-00 ·Pages 415-20

Traboni C, Ciliberto G, Cortese R

Abstract

We present a novel general method for localized mutagenesis. The DNA segment to be mutagenized is inserted in the beta-galactosidase gene of a M13-lac vector, generally causing loss of beta-galactosidase function by generation of frameshifts or nonsense codons. Mutations in the inserted DNA which restore beta-galactosidase function are readily detected and analyzed. The application of this method to the promoter of an eukaryotic (Caenorhabditis elegans) tRNAPro gene has allowed the isolation of several mutants altered in transcription.

MeSH Terms
Animals Base Sequence Caenorhabditis/genetics Coliphages/genetics Escherichia coli/genetics Female Genes Genes, Viral Genetic Vectors Mutation Oocytes/metabolism Operon RNA, Transfer, Amino Acyl/genetics Transcription, Genetic Xenopus beta-Galactosidase/genetics
Chemicals
RNA, Transfer, Amino Acyl beta-Galactosidase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Traboni C
Ciliberto G
Cortese R
References (35)
35 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1982-00-00
Pages
415-20
Language
English
Region
England
NLM ID
8208664
PMCID
PMC553061
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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