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PMID: 2410258 Published · ppublish English Journal Article

Analogue inhibitor of 2-5A action: effect on the interferon-mediated inhibition of encephalomyocarditis virus replication.

The EMBO journal ·Vol. 4 ·No. 2 ·1985-02-00 ·Pages 431-6

Watling D, Serafinowska HT, Reese CB, Kerr IM

Abstract

Chemically synthesised CH3Sp(A2'p)2A2'pp3'OCH3 has been used to assess the importance of the ppp(A2'p)nA (n greater than or equal to 2: 2-5A) system in the antiviral action of interferon against encephalomyocarditis virus (EMC). It inhibits activation of the 2-5A-dependent RNase by 2-5A in intact mouse L929 cells and cell-free systems. In interferon-treated, EMC-infected L929 cells it inhibits 2-5A-mediated rRNA cleavage and partially restores EMC RNA synthesis and virus yield. Activation of the 2-5A-dependent RNase must, therefore, play some part in interferon action against the growth of EMC virus in such cells.

MeSH Terms
Adenine Nucleotides/antagonists & inhibitors,pharmacology Animals Cell-Free System Encephalomyocarditis virus/growth & development Enzyme Activation/drug effects Interferons/antagonists & inhibitors L Cells Mice Oligonucleotides/antagonists & inhibitors,pharmacology Oligoribonucleotides/antagonists & inhibitors,pharmacology Protein Biosynthesis RNA, Ribosomal/metabolism Ribonucleases/metabolism Thionucleotides/pharmacology Virus Replication/drug effects
Chemicals
Adenine Nucleotides Oligonucleotides Oligoribonucleotides RNA, Ribosomal Thionucleotides 2',5'-oligoadenylate Interferons Ribonucleases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Watling D
Serafinowska H T
Reese C B
Kerr I M
References (25)
25 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1985-02-00
Pages
431-6
Language
English
Region
England
NLM ID
8208664
PMCID
PMC554204
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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