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PMID: 2410262 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Adenovirus VA RNAI mediates a translational stimulation which is not restricted to the viral mRNAs.

The EMBO journal ·Vol. 4 ·No. 4 ·1985-04-00 ·Pages 957-64

Svensson C, Akusjärvi G

Abstract

The effect of adenovirus VA RNAI on the translation of mRNAs expressing the bacterial chloramphenicol acetyltransferase (CAT) enzyme was studied by a transient expression assay in 293 cells. The CAT activity was determined in extracts prepared from cells transfected with mixtures of plasmids encoding CAT and VA RNA. The results showed that VA RNAI co-transfection resulted in a significant increase in CAT expression from a variety of constructs. Thus, expression of CAT from a SV40 mRNA, a beta-globin mRNA and a chimeric mRNA containing the adenovirus-2 tripartite leader were all stimulated approximately 6-fold by VA RNAI. Based on these results we conclude that the tripartite leader sequence is not required for the VA RNA-mediated stimulation of translation. Our results indicate instead that VA RNAI probably functions as a general enhancer of mRNA translation. A2- to 3-fold stimulation of CAT expression was also obtained following transient expression of HeLa and CV-1 cells. The reduced efficiency was correlated with a 10- to 20-fold lower level of VA RNA expression in HeLa compared with 293 cells. Thus, it is likely that a product from region E1 indirectly enhances the translational efficiency by stimulating VA RNA transcription.

MeSH Terms
Acetyltransferases/genetics Adenoviruses, Human/genetics Base Sequence Cell Line Chloramphenicol O-Acetyltransferase DNA/metabolism DNA Restriction Enzymes Genes, Viral Globins/genetics Humans Kidney/metabolism Plasmids Protein Biosynthesis RNA/genetics RNA, Messenger/genetics RNA, Neoplasm/genetics RNA, Small Nuclear Transcription, Genetic Transfection
Chemicals
RNA, Messenger RNA, Neoplasm RNA, Small Nuclear RNA Globins DNA Acetyltransferases Chloramphenicol O-Acetyltransferase DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Svensson C
Akusjärvi G
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49 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1985-04-00
Pages
957-64
Language
English
Region
England
NLM ID
8208664
PMCID
PMC554285
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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