Home LiteratureArticle Details
PMID: 24189656 Published · ppublish English Journal Article

Altered ribosomal protein L29 in a cycloheximide-resistant strain of Saccharomyces cerevisiae.

Current genetics ·Vol. 1 ·No. 3 ·1980-04-00 ·Pages 177-83

Stöcklein W, Piepersberg W

Abstract

A spontaneous high-level cycloheximide-resistant mutant of the yeast Saccharomyces cerevisiae (strain cy32) is found to have an altered protein of the large subunit (60S) of cytoplasmic ribosomes, namely protein L29. The resistance character segregates together with this biochemical defect and is semidominant in heterozygous diploids. Judged from in vitro susceptibility to inhibition by cycloheximide there are at least 50% resistant ribosomes present in such diploid strains. From these results it is concluded that cycloheximide resistance of mutant cy32 is caused by mutation of a single gene and that it is the structural gene for L29 which is affected.Preliminary genetic mapping data are also reported. They indicate a location of cyhx-32 marker on chromosome 7 near met13.

Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Stöcklein W
Lehrstuhl für Mikrobiologie der Universität, Universitätsstrasse 31, D-8400, Regensburg, Germany.
Piepersberg W
References (20)
20 references, click to expand
  1. Characterisation of the ribosomal proteins from Schizosaccharomyces pombe by two-dimensional polyacrylamide gel electrophoresis: demonstration that a cycloheximide resistant strain, cyh1, has an altered 60S ribosomal protein.
    Mol Gen Genet. 1977 Dec 14;158(1):93-100 PMID: 607151
  2. Cycloheximide resistance in Chinese hamster cells. III. Characterization of cell-free protein synthesis by polysomes.
    Mol Gen Genet. 1975;138(2):173-7 PMID: 1196299
  3. The ribosomal proteins of Escherichia coli. I. Purification of the 30S ribosomal proteins.
    Biochemistry. 1969 Jul;8(7):2897-905 PMID: 4897206
  4. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  5. A cycloheximide sensitivity factor from yeast required for N-acetylphenylalanylpuromycin formation.
    Biochemistry. 1976 Nov 2;15(22):4760-4 PMID: 791358
  6. Ribosomal proteins in the fungus Podospora anserina: evidence for an electrophoretically altered 60S protein in a cycloheximide resistant mutant.
    Mol Gen Genet. 1977 Nov 14;156(2):141-4 PMID: 600264
  7. Analysis of the protein composition of yeast ribosomal subunits by two-dimensional polyacrylamide gel electrophoresis.
    Mol Biol Rep. 1974 Sep;1(7):409-15 PMID: 4425489
  8. Initiation and elongation of protein synthesis in growing cells: differential inhibition by cycloheximide and emetine.
    Arch Biochem Biophys. 1977 Jul;182(1):171-80 PMID: 883830
  9. Cryptopleurine resistance: genetic locus for a 40S ribosomal component in Saccharomyces cerevisiae.
    J Bacteriol. 1974 Dec;120(3):1308-14 PMID: 4612014
  10. Yeast ribosomal proteins. I. Characterization of cytoplasmic ribosomal proteins by two-dimensional gel electrophoresis.
    Mol Gen Genet. 1978 Jul 4;162(3):259-68 PMID: 355832
  11. Cycloheximide resistance in yeast: a property of the 60s ribosomal subunit.
    Biochem Biophys Res Commun. 1967 Dec 15;29(5):696-704 PMID: 6077806
  12. Two-dimensional polyacrylamide gel electrophoresis: an improved method for ribosomal proteins.
    Anal Biochem. 1974 Jan;57(1):200-10 PMID: 4817496
  13. Genetics and biochemistry of cycloheximide resistance in Physarum polycephalum.
    Mol Gen Genet. 1972;118(2):97-107 PMID: 4562869
  14. GENETIC ANALYSIS OF ACTIDIONE RESISTANCE IN SACCHAROMYCES CEREVISIAE.
    Genet Res. 1965 Feb;6:130-8 PMID: 14297591
  15. Cycloheximide resistance can be mediated through either ribosomal subunit.
    Proc Natl Acad Sci U S A. 1978 Jul;75(7):3158-62 PMID: 277918
  16. Modified ribosomes conferring resistance to cycloheximide in mutants of Saccharomyces cerevisiae.
    J Mol Biol. 1967 Jun 14;26(2):347-50 PMID: 6034778
  17. Ribosomal proteins. VII. Two-dimensional polyacrylamide gel electrophoresis for fingerprinting of ribosomal proteins.
    Anal Biochem. 1970 Aug;36(2):401-12 PMID: 4916449
  18. Ribosomal proteins of yeast strains carrying mutations which affect the efficiency of nonsense suppression.
    Mol Gen Genet. 1978 Feb 16;159(2):223-5 PMID: 345095
  19. Role of ribosomes in cycloheximide resistance of Neurospora mutants.
    Mol Gen Genet. 1973 Aug 28;124(4):359-63 PMID: 4270517
  20. Selective inhibition of protein synthesis initiation in Saccharomyces cerevisiae by low concentrations of cycloheximide.
    J Biol Chem. 1976 Nov 25;251(22):7278-80 PMID: 791949
Article Info
Journal
Current genetics
Abbr.
Curr Genet
ISSN
0172-8083
DOI
10.1007/BF00390941
Published
1980-04-00
Pages
177-83
Language
English
Region
United States
NLM ID
8004904
External Links
PubMed source DOI: 10.1007/BF00390941 View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2