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PMID: 4612014 Published · ppublish English Journal Article

Cryptopleurine resistance: genetic locus for a 40S ribosomal component in Saccharomyces cerevisiae.

Journal of bacteriology ·Vol. 120 ·No. 3 ·1974-12-00 ·Pages 1308-14

Grant P, Sánchez L, Jiménez A

Abstract

Mutants resistant to the phenanthrene alkaloids tylophorine, tylocrebrine, and cryptopleurine have been isolated from the yeast Saccharomyces cerevisiae. A single recessive nuclear gene was responsible for resistance. This resistance locus, cry, was closely linked to the mating locus (2.2 centimorgans). The resistance was ribosomal and was due to an altered 40S ribosomal subunit. The mode of action of these drugs has been examined, and they appear to inhibit the translocation phase of protein synthesis.

MeSH Terms
Alkaloids/pharmacology Amino Acids/metabolism Breeding Carbon Radioisotopes Cell Fractionation Cell-Free System Chromosome Mapping Cycloheximide/pharmacology Drug Resistance, Microbial Fungal Proteins/biosynthesis Genes, Recessive Genetic Linkage Isoquinolines/pharmacology Mutation Phenanthrenes/pharmacology Phenylalanine/metabolism Poly U/metabolism Polyribosomes/drug effects,metabolism Quinolizines/pharmacology RNA, Messenger/metabolism Saccharomyces cerevisiae/drug effects,metabolism
Chemicals
Alkaloids Amino Acids Carbon Radioisotopes Fungal Proteins Isoquinolines Phenanthrenes Quinolizines RNA, Messenger Poly U cryptopleurine Phenylalanine Cycloheximide
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Grant P
Sánchez L
Jiménez A
References (21)
21 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.120.3.1308-1314.1974
Published
1974-12-00
Pages
1308-14
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC245916
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.120.3.1308-1314.1974 PMC Full Text View journal details
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