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PMID: 24189799 Published · ppublish English Journal Article

Isolation and characterization of mitochondrial DNA from the alkane yeast Saccharomycopsis lipolytica.

Current genetics ·Vol. 2 ·No. 2 ·1980-10-00 ·Pages 97-101

Kück U, Stahl U, Lhermitte A, Esser K

Abstract

Mitochondrial (mt) DNA of the alkane yeast, Saccharomycopsis lipolytica, was isolated. Its buoyant density in CsCl was found to be of 1.687 g/cm(3), indicating a GC content of 27.5% and its melting point Tm = 79.5 °C, indicating a GC content of 24.9%. The corresponding values for nuclear (n) DNA, are 1.709 g/cm(3) (GC: 49.5%) and Tm = 90.5 (GC: 51.7%) respectively. Electron microscopy revealed that mtDNA has a circular structure with a contour length of about 14.5 µm corresponding to 45.5 kb per molecule. The size estimated from restriction analyses performed with 7 endonucleases was 48.35 kb/molecule. A restriction map was constructed, using the cleavage data of 4 endonucleases.

Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kück U
Lehrstuhl für Allgemeine Botanik, Ruhr-Universität, Postfach 102148, D-4630, Bochum 1, Germany.
Stahl U
Lhermitte A
Esser K
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Article Info
Journal
Current genetics
Abbr.
Curr Genet
ISSN
0172-8083
DOI
10.1007/BF00420620
Published
1980-10-00
Pages
97-101
Language
English
Region
United States
NLM ID
8004904
External Links
PubMed source DOI: 10.1007/BF00420620 View journal details
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