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PMID: 24189910 Published · ppublish English Journal Article

Loss of 2 um DNA from Saccharomyces cerevisiae transformed with the chimaeric plasmid pJDB219.

Current genetics ·Vol. 2 ·No. 3 ·1980-12-00 ·Pages 201-5

Dobson MJ, Futcher AB, Cox BS

Abstract

Two plasmids containing Saccharomyces cerevisiae 2 µm DNA sequences and the S. cerevisiae LEU2 gene have been found to display incompatibility with 2 µm DNA; in the presence of the LEU2 plasmids, 2 µm DNA can be lost. The LEU2 plasmids can be lost spontaneously after (and before) 2 µm DNA loss has occurred, so that strains completely lacking 2 µm DNA sequences can be obtained routinely.

Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Dobson M J
Botany School, Sóuth Parks Road, 0X1 3RA, Oxford, England.
Futcher A B
Cox B S
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21 references, click to expand
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Article Info
Journal
Current genetics
Abbr.
Curr Genet
ISSN
0172-8083
DOI
10.1007/BF00435686
Published
1980-12-00
Pages
201-5
Language
English
Region
United States
NLM ID
8004904
External Links
PubMed source DOI: 10.1007/BF00435686 View journal details
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