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PMID: 24190052 Published · ppublish English Journal Article

Curing of Saccharomyces cerevisiae 2-μm DNA by transformation.

Current genetics ·Vol. 3 ·No. 2 ·1981-05-00 ·Pages 83-9

Erhart E, Hollenberg CP

Abstract

A general procedure for the curing of 2-μm in Saccharomyces cerevisiae is described. The method is based on the displacement of endogenous 2-μm DNA by the recombinant plasmid pMP78-1, which carries the yeast leu2 gene and the 2 -μm DNA replicon, but cannot be maintained stably in a yeast cell without endogenous 2-μm DNA. After transformation with pMP78-1 cells are grown selectively to displace 2-μm DNA. During the non-selective growth which follows, plasmid pMP78-1 is lost and up to 100% of the cells completely lack plasmids. In conjunction with a kanamycin resistance marker, as present in plasmid pMP81, this method should be applicable to cure any wild-type yeast strain. The stability of recombinant plasmids in cir (+) and cir (0) strains has been compared.

Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Erhart E
Institut für Mikrobiologie, Universität Düsseldorf, Universitätsstraße 1, D-4000, Düsseldorf, Germany.
Hollenberg C P
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Article Info
Journal
Current genetics
Abbr.
Curr Genet
ISSN
0172-8083
DOI
10.1007/BF00365710
Published
1981-05-00
Pages
83-9
Language
English
Region
United States
NLM ID
8004904
External Links
PubMed source DOI: 10.1007/BF00365710 View journal details
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