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PMID: 2421254 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Characterization of diverse forms of myosin heavy chain expressed in adult human skeletal muscle.

Nucleic acids research ·Vol. 14 ·No. 7 ·1986-04-11 ·Pages 2951-69

Saez L, Leinwand LA

Abstract

In an attempt to define myosin heavy chain (MHC) gene organization and expression in adult human skeletal muscle, we have isolated and characterized genomic sequences corresponding to different human sarcomeric MHC genes (1). In this report, we present the complete DNA sequence of two different adult human skeletal muscle MHC cDNA clones, one of which encodes the entire light meromyosin (LMM) segment of MHC and represents the longest described MHC cDNA sequence. Additionally, both clones provide new sequence data from a 228 amino acid segment of the MHC tail for which no protein or DNA sequence has been previously available. One clone encodes a "fast" form of skeletal muscle MHC while the other clone most closely resembles a MHC form described in rat cardiac ventricles. We show that the 3' untranslated region of skeletal MHC cDNAs are homologous from widely separated species as are cardiac MHC cDNAs. However, there is no homology between the 3' untranslated region of cardiac and skeletal muscle MHCs. Isotype-specific preservation of MHC 3' untranslated sequences during evolution suggests a functional role for these regions.

MeSH Terms
Adult Amino Acid Sequence Animals Base Sequence Chick Embryo Cloning, Molecular DNA/analysis DNA Restriction Enzymes/metabolism Gene Expression Regulation Humans Muscles/enzymology Myocardium/analysis Myosins/genetics Nucleic Acid Hybridization RNA/analysis Rats
Chemicals
RNA DNA DNA Restriction Enzymes Myosins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Saez L
Leinwand L A
References (37)
37 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/14.7.2951
Published
1986-04-11
Pages
2951-69
Language
English
Region
England
NLM ID
0411011
PMCID
PMC339714
Subset
IM
Grants
NIGMS NIH HHS · GM29090 · United States
Databases
GENBANK
X03740, X03741
External Links
PubMed source DOI: 10.1093/nar/14.7.2951 PMC Full Text View journal details
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