Novel class of rDNA repeat units in somatic hybrids between Nicotiana and Atropa.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik ·Vol. 76 ·No. 1 ·1988-07-00 ·Pages 108-12

Borisjuk NV, Momot VP, Gleba Y

Abstract

Behavior of ribosomal RNA genes in the process of somatic hybridization was analyzed using hybrids Nicotiana tabacum + Atropa belladonna. Blothybridization of parental species DNAs to (32)P-rDNA specific probes revealed two classes of ribosomal repeats in both tobacco and nightshade; their length was 11.2 kb, 10.4 kb (tobacco) and 9.4 kb, 10.2 kb (night-shade). For analysis of hybrids, labelled (32)P rDNA specific probes were hybridized to DNA of parental species and somatic hybrids digested with restriction endonucleases EcoR1, EcoRV and BamH1. A new class of ribosomal DNA repeat, absent in parental species, was found in hybrid line NtAb-1. Possible mechanisms of appearence of a new rDNA class in the process of somatic cell fusion are discussed.

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Borisjuk N V

Institute of Botany, Academy of Sciences of the Ukrainian SSR, Repina 2, 252601, Kiev, USSR.

Momot V P

Gleba Y

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Article Info

Journal

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik

Abbr.

Theor Appl Genet

ISSN

0040-5752

DOI

10.1007/BF00288839

Published

1988-07-00

Pages

108-12

Language

English

Region

Germany

NLM ID

0145600

External Links

PubMed source DOI: 10.1007/BF00288839 View journal details

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Novel class of rDNA repeat units in somatic hybrids between Nicotiana and Atropa.

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