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PMID: 2424011 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The neuronal identifier element is a cis-acting positive regulator of gene expression.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 11 ·1986-06-00 ·Pages 3751-5

McKinnon RD, Shinnick TM, Sutcliffe JG

Abstract

A middle-repetitive DNA element termed the identifier (ID) sequence, located in introns of postnatal-onset neuronal-specific genes, is transcribed in early postnatal rats by RNA polymerase III (Pol III) specifically in the brain. We show that these Pol III transcripts, although brain specific in vivo, are also expressed in immortalized rodent cell lines in culture. We demonstrate that the ID sequence can act as a positive regulator or enhancer of RNA polymerase II gene expression in cell lines that express these RNAs but not in primary cells in which Pol III transcription of the ID sequence is absent. Thus, ID elements may be positive regulators of postnatal-onset neuronal-specific gene expression.

MeSH Terms
Animals Cell Differentiation Cell Line DNA-Directed RNA Polymerases/metabolism Enhancer Elements, Genetic Gene Expression Regulation Genes, Regulator Genetic Linkage Mice Nerve Tissue Proteins/genetics Neurons/cytology,physiology RNA/genetics RNA Polymerase II/metabolism RNA Polymerase III/metabolism RNA, Small Cytoplasmic Rats Transcription, Genetic
Chemicals
Nerve Tissue Proteins RNA, Small Cytoplasmic RNA RNA Polymerase II DNA-Directed RNA Polymerases RNA Polymerase III
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
McKinnon R D
Shinnick T M
Sutcliffe J G
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37 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.11.3751
Published
1986-06-00
Pages
3751-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC323601
Subset
IM
Grants
NIGMS NIH HHS · GM32355 · United States
External Links
PubMed source DOI: 10.1073/pnas.83.11.3751 PMC Full Text View journal details
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