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PMID: 2428036 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Nucleic acid sequence of an internal image-bearing monoclonal anti-idiotype and its comparison to the sequence of the external antigen.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 17 ·1986-09-00 ·Pages 6578-82

Bruck C, Co MS, Slaoui M, Gaulton GN, Smith T, Fields BN, Mullins JI, Greene MI

Abstract

The monoclonal anti-idiotypic antibody (mAb2) 87.92.6 directed against the 9B.G5 antibody specific for the virus neutralizing epitope on the mammalian reovirus type 3 hemagglutinin was previously demonstrated to express an internal image of the receptor binding epitope of the reovirus type 3. Furthermore, this mAb2 has autoimmune reactivity to the cell surface receptor of the reovirus. The nucleotide and deduced amino acid sequences of the 87.92.6 mAb2 heavy and light chains are described in this report. The sequence analysis reveals that the same heavy chain variable and joining (VH and JH) gene segments are used by the 87.92.6 anti-idiotypic mAb2 and by the dominant idiotypes of the BALB/c anti-GAT (cGAT) and anti-NP (NPa) responses. [GAT; random polymer that is 60% glutamic acid, 30% alanine, and 10% tyrosine. NP; (4-hydroxy-3-nitrophenyl)-acetyl.] Despite extensive homology at the level of the heavy chain variable regions, the NPa positive BALB/c anti-NP monoclonal antibody 17.2.25 binds neither 9B.G5 nor the cellular receptor for the hemagglutinin. Amino acid sequence comparison between the viral hemagglutinin and the 87.92.6 mAb2 light chain "internal image," reveals an area of significant homology indicating that antigen mimicry by antibodies may be achieved by sharing primary structure.

MeSH Terms
Amino Acid Sequence Animals Antibodies, Anti-Idiotypic/genetics Antibodies, Monoclonal/genetics Base Sequence Cloning, Molecular Epitopes Hemagglutinins, Viral/genetics,immunology Immunoglobulin Idiotypes/immunology Immunoglobulin Variable Region/genetics Mice Nitrophenols/immunology Peptides/immunology Phenylacetates Polymers RNA, Messenger/genetics Reoviridae/immunology
Chemicals
Antibodies, Anti-Idiotypic Antibodies, Monoclonal Epitopes Hemagglutinins, Viral Immunoglobulin Idiotypes Immunoglobulin Variable Region Nitrophenols Peptides Phenylacetates Polymers RNA, Messenger 4-hydroxy-5-nitrophenyl acetic acid GAT
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Bruck C
Co M S
Slaoui M
Gaulton G N
Smith T
Fields B N
Mullins J I
Greene M I
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.17.6578
Published
1986-09-00
Pages
6578-82
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC386547
Subset
IM
Grants
NIADDK NIH HHS · 1R23-AM36837-01 · United States
NCI NIH HHS · CA 34975 · United States
NINDS NIH HHS · NS 16998-05 · United States
Databases
GENBANK
M13832, M13833
External Links
PubMed source DOI: 10.1073/pnas.83.17.6578 PMC Full Text View journal details
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